Characterization of the Genes Involved in Malic Acid
Metabolism from Pear Fruit and Their Expression Profile after Postharvest
1‑MCP/Ethrel Treatment
Libin Wang
Min Ma
Yanru Zhang
Zhangfei Wu
Lin Guo
Weiqi Luo
Li Wang
Zhen Zhang
Shaoling Zhang
10.1021/acs.jafc.8b02598.s001
https://acs.figshare.com/articles/dataset/Characterization_of_the_Genes_Involved_in_Malic_Acid_Metabolism_from_Pear_Fruit_and_Their_Expression_Profile_after_Postharvest_1_MCP_Ethrel_Treatment/6960884
In this study, five genes involved
in malic acid (MA) metabolism,
including a cytosolic NAD-dependent malate dehydrogenase gene (<i>cyNAD-MDH</i>), a cytosolic NADP-dependent malic enzyme gene
(<i>cyNADP-ME</i>), two vacuolar H<sup>+</sup>-ATPase genes
(<i>vVAtp1</i> and <i>vVAtp2</i>), and one vacuolar
inorganic pyrophosphatase gene (<i>vVPp</i>), were characterized
from pear fruit based on bioinformatic and experimental analysis.
Their expression profile in “Housui” pear was tissue-specific,
and their expression patterns during fruit development were diverse.
During “Housui” pear storage, MA content decreased,
which was associated with the downregulated transcripts of MA metabolism-related
genes and cyNAD-MDH activity and higher cyNADP-ME activity. The response
of MA metabolism to postharvest 1.5 μL L<sup>–1</sup> 1-MCP fumigation and 0.5 mL L<sup>–1</sup> ethrel dipping
was distinct: 1-MCP fumigation upregulated gene expression and cyNAD-MDH
activity and suppressed cyNADP-ME activity, and thus maintained higher
MA abundance when compared with those in the control; on the other
hand, an opposite behavior was observed in ethrel-treated fruit.
2018-08-03 00:00:00
1- MCP fumigation upregulated gene expression
MA metabolism-related genes
cytosolic NADP-dependent malic enzyme gene
cyNADP-ME activity
cytosolic NAD-dependent malate dehydrogenase gene
cyNAD-MDH activity