%0 Journal Article
%A Griffith, Corey
M.
%A Morgan, Melissa A.
%A Dinges, Meredith M.
%A Mathon, Caroline
%A Larive, Cynthia K.
%D 2018
%T Metabolic Profiling
of Chloroacetanilide Herbicides
in Earthworm Coelomic Fluid Using 1H NMR and GC–MS
%U https://acs.figshare.com/articles/journal_contribution/Metabolic_Profiling_of_Chloroacetanilide_Herbicides_in_Earthworm_Coelomic_Fluid_Using_sup_1_sup_H_NMR_and_GC_MS/6820895
%R 10.1021/acs.jproteome.8b00081.s001
%2 https://acs.figshare.com/ndownloader/files/12404786
%K GC
%K earthworm coelomic fluid
%K S-metolachlor
%K 1 H NMR
%K tricarboxylic acid cycle
%K monitoring earthworm metabolism
%K Earthworm Coelomic Fluid
%K perturbation
%K chloroacetanilide herbicides
%K MB-OPLS-DA
%K analysis
%K racemic metolachlor
%X Earthworms
(Eisenia fetida) are vital members
of the soil environment. Because of their sensitivity to many contaminants,
monitoring earthworm metabolism may be a useful indicator of environmental
stressors. Here, metabolic profiles of exposure to five chloroacetanilide
herbicides and one enantiomer (acetochlor, alachlor, butachlor, racemic
metolachlor, S-metolachlor, and propachlor) are observed in earthworm
coelomic fluid using proton nuclear magnetic resonance spectroscopy
(NMR) and gas chromatography–mass spectrometry (GC–MS).
Multiblocked-orthogonal partial least-squares-discriminant analysis
(MB-OPLS-DA) and univariate analysis were used to identify metabolic
perturbations in carnitine biosynthesis, carbohydrate metabolism,
lipid metabolism, nitrogen metabolism, and the tricarboxylic acid
cycle. Intriguingly, stereospecific metabolic responses were observed
between racemic metolachlor and S-metolachlor exposed worms. These
findings support the utility of coelomic fluid in monitoring metabolic
perturbations induced by chloroacetanilide herbicides in nontarget
organisms and reveal specificity in the metabolic impacts of herbicide
analogues in earthworms.
%I ACS Publications