Implementation of Fragment Ion Protection (FIP) during
Ultraviolet Photodissociation (UVPD) Mass Spectrometry
Dustin
D. Holden
James D. Sanders
Chad R. Weisbrod
Christopher Mullen
Jae C. Schwartz
Jennifer S. Brodbelt
10.1021/acs.analchem.8b01723.s001
https://acs.figshare.com/articles/journal_contribution/Implementation_of_Fragment_Ion_Protection_FIP_during_Ultraviolet_Photodissociation_UVPD_Mass_Spectrometry/6771680
Ultraviolet
photodissociation (UVPD) is a nonselective activation
method in which both precursor and fragment ions may absorb photons
and dissociate. Photoactivation of fragment ions may result in secondary
or multiple generations of dissociation, which decreases the signal-to-noise
ratio (S/N) of larger fragment ions owing to the prevalent subdivision
of the ion current into many smaller, often less informative, fragment
ions. Here we report the use of dipolar excitation waveforms to displace
fragment ions out of the laser beam path, thus alleviating the extent
of secondary dissociation during 193 nm UVPD. This fragment ion protection
(FIP) strategy increases S/N of larger fragment ions and improves
the sequence coverage obtained for proteins via retaining information
deeper into the midsection of protein sequences.
2018-06-21 00:00:00
Fragment Ion Protection
FIP
fragment ion protection
nonselective activation method
fragment ions
laser beam path
193 nm UVPD
dipolar excitation waveforms
Mass Spectrometry Ultraviolet photodissociation