Spiral Layer-by-Layer Micro-Nanostructured Scaffolds
for Bone Tissue Engineering
Ohan S. Manoukian
Aja Aravamudhan
Paul Lee
Michael R. Arul
Xiaojun Yu
Swetha Rudraiah
Sangamesh G. Kumbar
10.1021/acsbiomaterials.8b00393.s001
https://acs.figshare.com/articles/journal_contribution/Spiral_Layer-by-Layer_Micro-Nanostructured_Scaffolds_for_Bone_Tissue_Engineering/6222458
This
Article reports the fabrication and characterization of composite
micro-nanostructured spiral scaffolds functionalized with nanofibers
and hydroxyapatite (HA) for bone regeneration. The spiral poly(lactic
acid-<i>co</i>-glycolic acid) (PLGA) porous microstructure
was coated with sparsely spaced PLGA nanofibers and HA to enhance
surface area and bioactivity. Polyelectrolyte-based HA coating in
a layer-by-layer (LBL) fashion allowed 10–70 μM Ca<sup>2+</sup>/mm<sup>2</sup> incorporation. These scaffolds provided a
controlled release of Ca<sup>2+</sup> ions up to 60 days with varied
release kinetics accounting up to 10–50 μg. Spiral scaffolds
supported superior adhesion, proliferation, and osteogenic differentiation
of rat bone marrow stromal cells (MSCs) as compared to controls microstructures.
Spiral micro-nanostructures supported homogeneous tissue ingrowth
and resulted in bone-island formation in the center of the scaffold
as early as 3 weeks in a rabbit ulnar bone defect model. In contrast,
control cylindrical scaffolds showed tissue ingrowth only at the surface
because of limitations in scaffold transport features.
2018-04-25 00:00:00
Polyelectrolyte-based HA coating
release kinetics accounting
scaffold transport features
rat bone marrow stromal cells
Spiral Layer-by-Layer Micro-Nanostructured Scaffolds
tissue ingrowth
micro-nanostructured spiral scaffolds functionalized
PLGA
rabbit ulnar bone defect model
LBL
Bone Tissue Engineering
MSC