Cell-Free and <i>In Vivo</i> Characterization
of Lux, Las, and Rpa Quorum Activation Systems in <i>E. coli</i>
Andrew D. Halleran
Richard M. Murray
10.1021/acssynbio.7b00376.s001
https://acs.figshare.com/articles/journal_contribution/Cell-Free_and_i_In_Vivo_i_Characterization_of_Lux_Las_and_Rpa_Quorum_Activation_Systems_in_i_E_coli_i_/5640436
Synthetic
biologists have turned toward quorum systems as a path for building
sophisticated microbial consortia that exhibit group decision making.
Currently, however, even the most complex consortium circuits rely
on only one or two quorum sensing systems, greatly restricting the
available design space. High-throughput characterization of available
quorum sensing systems is useful for finding compatible sets of systems
that are suitable for a defined circuit architecture. Recently, cell-free
systems have gained popularity as a test-bed for rapid prototyping
of genetic circuitry. We take advantage of the transcription–translation
cell-free system to characterize three commonly used Lux-type quorum
activators, Lux, Las, and Rpa. We then compare the cell-free characterization
to results obtained <i>in vivo</i>. We find significant
genetic crosstalk in both the Las and Rpa systems and substantial
signal crosstalk in Lux activation. We show that cell-free characterization
predicts crosstalk observed <i>in vivo</i>.
2017-11-09 00:00:00
Rpa systems
Vivo Characterization
design space
consortium circuits
Rpa Quorum Activation Systems
High-throughput characterization
E . coli
Las
signal crosstalk
Lux-type quorum activators
exhibit group decision
quorum systems
. coli
circuit architecture