%0 Journal Article
%A Karavasili, Christina
%A Komnenou, Anastasia
%A L. Katsamenis, Orestis
%A Charalampidou, Glykeria
%A Kofidou, Evangelia
%A Andreadis, Dimitrios
%A Koutsopoulos, Sotirios
%A Fatouros, Dimitrios G.
%D 2017
%T Self-Assembling Peptide Nanofiber Hydrogels for Controlled
Ocular Delivery of Timolol Maleate
%U https://acs.figshare.com/articles/journal_contribution/Self-Assembling_Peptide_Nanofiber_Hydrogels_for_Controlled_Ocular_Delivery_of_Timolol_Maleate/5605735
%R 10.1021/acsbiomaterials.7b00706.s001
%2 https://acs.figshare.com/ndownloader/files/9754921
%K vivo permeation studies
%K AUC
%K I-CONH
%K drug solution
%K vivo studies
%K self-assembling peptide sequence
%K CONH 2 hydrogel
%K electron microscopy studies
%K Timolol Maleate
%K Controlled Ocular Delivery
%K 24 h
%K self-assembling peptide hydrogels
%K CONH 2 peptide
%K Self-Assembling Peptide Nanofiber Hydrogels
%K β- blocker timolol maleate
%K form hydrogels
%K Ac
%K rheological studies
%K CONH 2
%K drug delivery
%K drug-loaded hydrogels
%K Histological studies
%X The
self-assembling peptides Ac-(RADA)4-CONH2 and
Ac-(IEIK)3I-CONH2, which form hydrogels
in physiological conditions, were evaluated as carriers for ocular
delivery of the β-blocker timolol maleate. Electron microscopy
studies revealed that hydrogels contain nanofibers, whereas rheological
studies showed that the Ac-(IEIK)3I-CONH2 self-assembles
in a stiffer hydrogel compared with the Ac-(RADA)4-CONH2 peptide. The in vitro release and ex vivo permeation studies
demonstrated controlled release and transport of the drug through
the cornea, which depended on the self-assembling peptide sequence.
In vivo studies in rabbits showed significant increase in the area
under the concentration–time curve (AUC) after administration
of the drug through the Ac-(RADA)4-CONH2 hydrogel
compared to drug solution, whereas a sustained reduction of intraocular
pressure for up to 24 h after instillation was achieved for both drug-loaded
hydrogels. Histological studies revealed good ocular tolerability
upon application of the formulations, suggesting that self-assembling
peptide hydrogels are promising systems for sustained ocular drug
delivery.
%I ACS Publications