%0 Journal Article
%A Olivares, Christopher
I.
%A Madeira, Camila L.
%A Sierra-Alvarez, Reyes
%A Kadoya, Warren
%A Abrell, Leif
%A Chorover, Jon
%A Field, Jim A.
%D 2017
%T Environmental
Fate of 14C Radiolabeled
2,4-Dinitroanisole in Soil Microcosms
%U https://acs.figshare.com/articles/journal_contribution/Environmental_Fate_of_sup_14_sup_C_Radiolabeled_2_4-Dinitroanisole_in_Soil_Microcosms/5571712
%R 10.1021/acs.est.7b03699.s001
%2 https://acs.figshare.com/ndownloader/files/9680473
%K nonextractable soil humus fraction
%K electron donor amendments
%K Irreversible humin incorporation
%K extracellular oxidoreductase enzyme horseradish peroxidase
%K humin fraction
%K 14 C-DNAN incorporation
%K HRP
%K remediation strategy
%K OC
%K DNAN biotransformation products
%K 14 C-DNAN
%X 2,4-Dinitrosanisole
(DNAN) is an insensitive munitions component
replacing conventional explosives. While DNAN is known to biotransform
in soils to aromatic amines and azo-dimers, it is seldom mineralized
by indigenous soil bacteria. Incorporation of DNAN biotransformation
products into soil as humus-bound material could serve as a plausible
remediation strategy. The present work studied biotransformation of
DNAN in soil and sludge microcosms supplemented with uniformly ring-labeled 14C-DNAN to quantify the distribution of label in soil, aqueous,
and gaseous phases. Electron donor amendments, different redox conditions
(anaerobic, aerobic, sequential anaerobic–aerobic), and the
extracellular oxidoreductase enzyme horseradish peroxidase (HRP) were
evaluated to maximize incorporation of DNAN biotransformation products
into the nonextractable soil humus fraction, humin. Irreversible humin
incorporation of 14C-DNAN occurred at higher rates in anaerobic
conditions, with a moderate increase when pyruvate was added. Additionally,
a single dose of HRP resulted in an instantaneous increased incorporation
of 14C-DNAN into the humin fraction. 14C-DNAN
incorporation to the humin fraction was strongly correlated (R2 = 0.93) by the soil organic carbon (OC) amount
present (either intrinsic or amended). Globally, our results suggest
that DNAN biotransformation products can be irreversibly bound to
humin in soils as a remediation strategy, which can be enhanced by
adding soil OC.
%I ACS Publications