Alternative SiO<sub>2</sub> Surface Direct MDCK Epithelial
Behavior
Alan D. Covell
Zheng Zeng
Taylor Mabe
Jianjun Wei
Amy Adamson
Dennis R. LaJeunesse
10.1021/acsbiomaterials.7b00645.s001
https://acs.figshare.com/articles/journal_contribution/Alternative_SiO_sub_2_sub_Surface_Direct_MDCK_Epithelial_Behavior/5552533
The
mechanical interactions of cells are mediated through adhesive
interactions. In this study, we examined the growth, cellular behavior,
and adhesion of MDCK epithelial cells on three different SiO<sub>2</sub> substrates: amorphous glass coverslips and the silicon oxide layers
that grow on ⟨111⟩ and ⟨100⟩ wafers. While
compositionally all three substrates are almost similar, differences
in surface energy result in dramatic differences in epithelial cell
morphology, cell–cell adhesion, cell–substrate adhesion,
actin organization, and extracellular matrix (ECM) protein expression.
We also observe striking differences in ECM protein binding to the
various substrates due to the hydrogen bond interactions. Our results
demonstrate that MDCK cells have a robust response to differences
in substrates that is not obviated by nanotopography or surface composition
and that a cell’s response may manifest through subtle differences
in surface energies of the materials. This work strongly suggests
that other properties of a material other than composition and topology
should be considered when interpreting and controlling interactions
of cells with a substrate, whether it is synthetic or natural.
2017-10-12 00:00:00
protein expression
MDCK Epithelial Behavior
MDCK epithelial cells
epithelial cell morphology
MDCK cells
Alternative SiO 2 Surface
adhesion
actin organization
surface energy result
surface composition
surface energies
silicon oxide layers
hydrogen bond interactions
extracellular matrix
ECM protein binding
glass coverslips
SiO 2 substrates