10.1021/acs.jproteome.7b00353.s001
Siqi Li
Siqi
Li
Yanbin He
Yanbin
He
Zhilong Lin
Zhilong
Lin
Shaohang Xu
Shaohang
Xu
Ruo Zhou
Ruo
Zhou
Feng Liang
Feng
Liang
Jian Wang
Jian
Wang
Huanming Yang
Huanming
Yang
Siqi Liu
Siqi
Liu
Yan Ren
Yan
Ren
Digging More Missing
Proteins Using an Enrichment
Approach with ProteoMiner
American Chemical Society
2017
protein detection
low-abundance proteins benefits MPs
Missing Proteins
ProteoMiner enrichment
ProteoMiner Human Proteome Project
PE
nucleus proteins
low-abundance proteins
HPP
Enrichment Approach
peptide fractionation
PRM assay
20 MPs
equalizing technique
high-abundance proteins
2017-09-29 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Digging_More_Missing_Proteins_Using_an_Enrichment_Approach_with_ProteoMiner/5544214
Human
Proteome Project (HPP) aims at mapping entire human proteins
with a systematic effort upon all the emerging techniques, which would
enhance understanding of human biology and lay a foundation for development
of medical applications. Until now, 2563 missing proteins (MPs, PE2–4)
are still undetected even using the most sensitive approach of protein
detection. Herein, we propose that enrichment of low-abundance proteins
benefits MPs finding. ProteoMiner is an equalizing technique by reducing
high-abundance proteins and enriching low-abundance proteins in biological
liquids. With triton X-100/TBS buffer extraction, ProteoMiner enrichment,
and peptide fractionation, 20 MPs (at least two non-nested unique
peptides with more than eight a.a. length) with 60 unique peptides
were identified from four human tissues including eight membrane/secreted
proteins and five nucleus proteins. Then 15 of them were confirmed
with two non-nested unique peptides (≥9 a.a.) identified
by matching well with their chemically synthetic peptides in PRM assay.
Hence, these results demonstrated ProteoMiner as a powerful means
in discovery of MPs.