Elucidating <i>Escherichia coli</i> Proteoform
Families Using Intact-Mass Proteomics and a Global PTM Discovery Database
Yunxiang Dai
Michael R. Shortreed
Mark Scalf
Brian L. Frey
Anthony J. Cesnik
Stefan Solntsev
Leah V. Schaffer
Lloyd M. Smith
10.1021/acs.jproteome.7b00516.s002
https://acs.figshare.com/articles/dataset/Elucidating_i_Escherichia_coli_i_Proteoform_Families_Using_Intact-Mass_Proteomics_and_a_Global_PTM_Discovery_Database/5503933
A proteoform family is a group of
related molecular forms of a
protein (proteoforms) derived from the same gene. We have previously
described a strategy to identify proteoforms and elucidate proteoform
families in complex mixtures of intact proteins. The strategy is based
upon measurements of two properties for each proteoform: (i) the accurate
proteoform intact-mass, measured by liquid chromatography/mass spectrometry
(LC–MS), and (ii) the number of lysine residues in each proteoform,
determined using an isotopic labeling approach. These measured properties
are then compared with those extracted from a catalog of theoretical
proteoforms containing protein sequences and localized post-translational
modifications (PTMs) for the organism under study. A match between
the measured properties and those in the catalog constitutes an identification
of the proteoform. In the present study, this strategy is extended
by utilizing a global PTM discovery database and is applied to the
widely studied model organism <i>Escherichia coli</i>, providing
the most comprehensive elucidation of <i>E. coli</i> proteoforms
and proteoform families to date.
2017-10-02 00:00:00
Elucidating Escherichia coli Proteoform Families
model organism Escherichia coli
lysine residues
proteoform families
protein sequences
coli proteoforms
Intact-Mass Proteomics
PTM discovery database
LC
post-translational modifications
proteoform intact-mass
proteoform family
Global PTM Discovery Database
elucidate proteoform families