Three-Dimensional
Super-resolution Imaging of Single
Nanoparticles Delivered by Pipettes
Yun Yu
Vignesh Sundaresan
Sabyasachi Bandyopadhyay
Yulun Zhang
Martin A. Edwards
Kim McKelvey
Henry S. White
Katherine A. Willets
10.1021/acsnano.7b05902.s004
https://acs.figshare.com/articles/journal_contribution/Three-Dimensional_Super-resolution_Imaging_of_Single_Nanoparticles_Delivered_by_Pipettes/5472739
Controlled three-dimensional
positioning of nanoparticles is achieved
by delivering single fluorescent nanoparticles from a nanopipette
and capturing them at well-defined regions of an electrified substrate.
To control the position of single nanoparticles, the force of the
pressure-driven flow from the pipette is balanced by the attractive
electrostatic force at the substrate, providing a strategy by which
nanoparticle trajectories can be manipulated in real time. To visualize
nanoparticle motion, a resistive-pulse electrochemical setup is coupled
with an optical microscope, and nanoparticle trajectories are tracked
in three dimensions using super-resolution fluorescence imaging to
obtain positional information with precision in the tens of nanometers.
As the particles approach the substrate, the diffusion kinetics are
analyzed and reveal either subdiffusive (hindered) or superdiffusive
(directed) motion depending on the electric field at the substrate
and the pressure-driven flow from the pipette. By balancing the effects
of the forces exerted on the particle by the pressure and electric
fields, controlled, real-time manipulation of single nanoparticle
trajectories is achieved. The developed approach has implications
for a variety of applications such as surface patterning and drug
delivery using colloidal nanoparticles.
2017-10-02 00:00:00
Single Nanoparticles Delivered
Three-Dimensional Super-resolution Imaging
resistive-pulse electrochemical setup
pressure-driven flow
super-resolution fluorescence imaging
substrate
nanoparticle trajectories