%0 Journal Article %A Dai, Chun-Guang %A Wang, Ji-Long %A Fu, Ying-Long %A Zhou, Hong-Ping %A Song, Qin-Hua %D 2017 %T Selective and Real-Time Detection of Nitric Oxide by a Two-Photon Fluorescent Probe in Live Cells and Tissue Slices %U https://acs.figshare.com/articles/journal_contribution/Selective_and_Real-Time_Detection_of_Nitric_Oxide_by_a_Two-Photon_Fluorescent_Probe_in_Live_Cells_and_Tissue_Slices/5434912 %R 10.1021/acs.analchem.7b02680.s001 %2 https://acs.figshare.com/ndownloader/files/9387451 %K GM %K exhibits intramolecular charge transfer %K detection %K probe NO-QA 5 exhibits %K Tissue Slices Nitric oxide %K diazonium %K NO-QA 5 %K nM %K LOD %K response %K two-photon excitation %K ICT %K nonfluorescent NO-QA 5 %X Nitric oxide (NO) is an important signaling molecule involved in many physiological and pathological processes. To understand these NO-mediated processes, it is a key to develop rapid and specific detection methods for NO. In the past 2 decades, numerous excellent fluorescent probes for NO have been designed; however, it still remains limitations such as slow response, low selectivity, and short excitation wavelength (<600 nm). In this Article, a two-photon fluorescent probe, NO-QA5, has been developed with 3-dimethylaminophenyl linking at the 6-position of 5-aminoquinoline as both the active site and prefluorophore for detection of NO. The nonfluorescent NO-QA5 can fast react with NO via a diazonium intermediate to generate two azoic regioisomers, one of which exhibits intramolecular charge transfer (ICT) emission, and two-photon absorption behavior (δΦ = 57 GM), giving a turn-on fluorescence rapid response. The sensing reaction is pH-insensitive in the range of 6–11 and highly selective and well sensitive (LOD = 15 nM), possible undergoing the same intermediate diazonium with the reaction under diazotization condition (NaNO2/HCl). Also, as a nitrite fluorescent probe NO-QA5 exhibits highly sensitive (LOD = 7 nM). Therefore, NO-QA5 can serve as a dual functional fluorescent probe for NO and NO2. Furthermore, NO-QA5 as a specific imaging agent has been demonstrated by achieving both exogenous and endogenous detections of NO in living cells under both one- and two-photon excitation and high resolution in tissue slices under two-photon excitation. %I ACS Publications