Hydrogen Sulfide and Reactive Sulfur Species Impact
Proteome <i>S</i>‑Sulfhydration and Global Virulence
Regulation in <i>Staphylococcus aureus</i>
Hui Peng
Yixiang Zhang
Lauren D. Palmer
Thomas E. Kehl-Fie
Eric P. Skaar
Jonathan C. Trinidad
David P. Giedroc
10.1021/acsinfecdis.7b00090.s002
https://acs.figshare.com/articles/dataset/Hydrogen_Sulfide_and_Reactive_Sulfur_Species_Impact_Proteome_i_S_i_Sulfhydration_and_Global_Virulence_Regulation_in_i_Staphylococcus_aureus_i_/5382367
Hydrogen
sulfide (H<sub>2</sub>S) is thought to protect bacteria from oxidative
stress, but a comprehensive understanding of its function in bacteria
is largely unexplored. In this study, we show that the human pathogen <i>Staphylococcus aureus</i> (<i>S. aureus</i>)
harbors significant effector molecules of H<sub>2</sub>S signaling,
reactive sulfur species (RSS), as low molecular weight persulfides
of bacillithiol, coenzyme A, and cysteine, and significant inorganic
polysulfide species. We find that proteome <i>S</i>-sulfhydration,
a post-translational modification (PTM) in H<sub>2</sub>S signaling,
is widespread in <i>S. aureus</i>. RSS levels modulate
the expression of secreted virulence factors and the cytotoxicity
of the secretome, consistent with an <i>S</i>-sulfhydration-dependent
inhibition of DNA binding by MgrA, a global virulence regulator. Two
previously uncharacterized thioredoxin-like proteins, denoted TrxP
and TrxQ, are <i>S</i>-sulfhydrated in sulfide-stressed
cells and are capable of reducing protein hydrodisulfides, suggesting
that this PTM is potentially regulatory in <i>S. aureus</i>. In conclusion, our results reveal that <i>S. aureus</i> harbors a pool of proteome- and metabolite-derived RSS capable of
impacting protein activities and gene regulation and that H<sub>2</sub>S signaling can be sensed by global regulators to affect the expression
of virulence factors.
2017-08-29 00:00:00
. aureu
S . aureus
virulence factors
bacteria
PTM
uncharacterized thioredoxin-like proteins
expression
DNA
H 2 S
Global Virulence Regulation
regulator
Staphylococcus aureus Hydrogen sulfide
impacting protein activities
S . aureus harbors
pathogen Staphylococcus aureus
proteome
reactive sulfur species
RSS