Marty, Florian Rago, Gianluca Smith, Donald F. Gao, Xiaoli Eijkel, Gert B. MacAleese, Luke Bonn, Mischa Brunner, Erich Basler, Konrad M. A. Heeren, Ron Combining Time-of-Flight Secondary Ion Mass Spectrometry Imaging Mass Spectrometry and CARS Microspectroscopy Reveals Lipid Patterns Reminiscent of Gene Expression Patterns in the Wing Imaginal Disc of <i>Drosophila melanogaster</i> Using label-free ToF-SIMS imaging mass spectrometry, we generated a map of small molecules differentially expressed in the <i>Drosophila</i> wing imaginal disc. The distributions of these moieties were in line with gene expression patterns observed during wing imaginal disc development. Combining ToF-SIMS imaging and coherent anti-Stokes Raman spectroscopy (CARS) microspectroscopy allowed us to locally identify acylglycerols as the main constituents of the pattern differentiating the future body wall tissue from the wing blade tissue. The findings presented herein clearly demonstrate that lipid localization patterns are strongly correlated with a developmental gene expression. From this correlation, we hypothesize that lipids play a so far unrecognized role in organ development. lipid localization patterns;Gene Expression Patterns;organ development;molecules differentially;Drosophila wing imaginal disc;Wing Imaginal Disc;Drosophila melanogaster;Ion Mass Spectrometry Imaging Mass Spectrometry;ToF-SIMS imaging;anti-Stokes Raman spectroscopy;gene expression patterns;label-free ToF-SIMS imaging mass spectrometry;wing imaginal disc development;future body wall tissue;wing blade tissue;Lipid Patterns Reminiscent;CARS Microspectroscopy;gene expression 2017-07-20
    https://acs.figshare.com/articles/journal_contribution/Combining_Time-of-Flight_Secondary_Ion_Mass_Spectrometry_Imaging_Mass_Spectrometry_and_CARS_Microspectroscopy_Reveals_Lipid_Patterns_Reminiscent_of_Gene_Expression_Patterns_in_the_Wing_Imaginal_Disc_of_i_Drosophila_melanogaster_i_/5353723
10.1021/acs.analchem.7b00125.s001