10.1021/acs.jnatprod.6b00730.s002
Chonticha Seeka
Chonticha
Seeka
Samran Prabpai
Samran
Prabpai
Palangpon Kongsaeree
Palangpon
Kongsaeree
Supinya Tewtrakul
Supinya
Tewtrakul
Thitima Lhinhatrakool
Thitima
Lhinhatrakool
Somyote Sutthivaiyakit
Somyote
Sutthivaiyakit
Anti-inflammatory 12,20-Epoxypregnane and 11,12-<i>seco</i>-Pregnane Glycosides from the Stems of <i>Hoya
kerrii</i>
American Chemical Society
2017
motif
skeleton
COX
anti-inflammatory
dose-dependent
ROESY
Epoxypregnane
Glycoside
RAW 264.7 cells
96.5 μ M
mechanism
experiment
Stems
epoxypregnane
IC 50 values
pregnane
inhibition
Pregnane
compound
down-regulate mRNA expression
analyses
configuration
X-ray
glycosides
iNOS
seco
Anti-inflammatory
spirodilactone cleavage products 8
Hoya kerrii
-2
manner
12.6
2017-05-31 13:50:26
Dataset
https://acs.figshare.com/articles/dataset/Anti-inflammatory_12_20-Epoxypregnane_and_11_12-_i_seco_i_-Pregnane_Glycosides_from_the_Stems_of_i_Hoya_kerrii_i_/5053708
Five 12,20-epoxypregnane glycosides
(<b>1</b>–<b>3</b>, <b>5</b>, and <b>6</b>) and two 11,12-<i>seco</i>-pregnane glycosides (<b>4</b> and <b>7</b>) with spirodilactone motifs, as well
as spirodilactone cleavage products <b>8</b> and <b>9</b>, were isolated from the stems of <i>Hoya kerrii</i>. The
relative configurations of the three related skeletons were supported
by ROESY experiments and X-ray crystallographic analyses. The isolates
were evaluated for their anti-inflammatory activity based on the inhibition
of NO production in RAW264.7 cells, and some showed IC<sub>50</sub> values ranging from 12.6 to 96.5 μM. The most potent compound, <b>9a</b>, was also examined for its anti-inflammatory mechanism
against mRNA expression and was found to down-regulate mRNA expression
of iNOS and COX-2 in a dose-dependent manner.