Seeka, Chonticha Prabpai, Samran Kongsaeree, Palangpon Tewtrakul, Supinya Lhinhatrakool, Thitima Sutthivaiyakit, Somyote Anti-inflammatory 12,20-Epoxypregnane and 11,12-<i>seco</i>-Pregnane Glycosides from the Stems of <i>Hoya kerrii</i> Five 12,20-epoxypregnane glycosides (<b>1</b>–<b>3</b>, <b>5</b>, and <b>6</b>) and two 11,12-<i>seco</i>-pregnane glycosides (<b>4</b> and <b>7</b>) with spirodilactone motifs, as well as spirodilactone cleavage products <b>8</b> and <b>9</b>, were isolated from the stems of <i>Hoya kerrii</i>. The relative configurations of the three related skeletons were supported by ROESY experiments and X-ray crystallographic analyses. The isolates were evaluated for their anti-inflammatory activity based on the inhibition of NO production in RAW264.7 cells, and some showed IC<sub>50</sub> values ranging from 12.6 to 96.5 μM. The most potent compound, <b>9a</b>, was also examined for its anti-inflammatory mechanism against mRNA expression and was found to down-regulate mRNA expression of iNOS and COX-2 in a dose-dependent manner. motif;skeleton;COX;anti-inflammatory;dose-dependent;ROESY;Epoxypregnane;Glycoside;RAW 264.7 cells;96.5 μ M;mechanism;experiment;Stems;epoxypregnane;IC 50 values;pregnane;inhibition;Pregnane;compound;down-regulate mRNA expression;analyses;configuration;X-ray;glycosides;iNOS;seco;Anti-inflammatory;spirodilactone cleavage products 8;Hoya kerrii;-2;manner;12.6 2017-05-31
    https://acs.figshare.com/articles/dataset/Anti-inflammatory_12_20-Epoxypregnane_and_11_12-_i_seco_i_-Pregnane_Glycosides_from_the_Stems_of_i_Hoya_kerrii_i_/5053705
10.1021/acs.jnatprod.6b00730.s003