10.1021/acs.est.7b00826.s001
Leilei Bai
Leilei
Bai
Chicheng Cao
Chicheng
Cao
Changhui Wang
Changhui
Wang
Huacheng Xu
Huacheng
Xu
Hui Zhang
Hui
Zhang
Vera I. Slaveykova
Vera I.
Slaveykova
Helong Jiang
Helong
Jiang
Toward
Quantitative Understanding of the Bioavailability
of Dissolved Organic Matter in Freshwater Lake during Cyanobacteria
Blooming
American Chemical Society
2017
insight
laboratory 4- stage plug-flow bioreactor
Cyanobacteria Blooming Occurrence
FTICR-MS
eutrophic Lake Taihu
carboxylic-rich alicyclic compounds
component
DOM
bioavailability
Dissolved Organic Matter
ultrahigh resolution mass spectrometry
molecule
biogeochemical processes
2017-05-03 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Toward_Quantitative_Understanding_of_the_Bioavailability_of_Dissolved_Organic_Matter_in_Freshwater_Lake_during_Cyanobacteria_Blooming/4996730
Occurrence of cyanobacterial harmful
algal blooms (CyanoHAB) can
induce considerable patchiness in the concentration and bioavailability
of dissolved organic matter (DOM), which could influence biogeochemical
processes and fuel microbial metabolism. In the present study, a laboratory
4-stage plug-flow bioreactor was used to successfully separate the
CyanoHAB-derived DOM isolated from the eutrophic Lake Taihu (China)
into continuum classes of bioavailable compounds. A combination of
new state-of-the-art tools borrowed from analytical chemistry and
microbial ecology were used to characterize quantitatively the temporary
evolution of DOM and to get deeper insights into its bioavailability.
The results showed a total 79% dissolved organic carbon loss over
time accompanied by depletion of protein-like fluorescent components,
especially the relatively hydrophilic ones. However, hydrophilic humic-like
fluorescent components exhibited bioresistant behavior. Consistently,
ultrahigh resolution mass spectrometry (FTICR-MS) revealed that smaller,
less aromatic, more oxygenated, and nitrogen-rich molecules were preferentially
consumed by microorganisms with the production of lipid-like species,
whereas recalcitrant molecules were primarily composed of carboxylic-rich
alicyclic compounds. Moreover, the bioavailability of DOM was negatively
correlated with microbial community diversity in the bioreactor. Results
from this study provide deeper insights into the fate of DOM and relevant
biogeochemical processes in eutrophic lakes.