10.1021/acssensors.7b00210.s001
Golbarg
M. Roozbahani
Golbarg
M.
Roozbahani
Xiaohan Chen
Xiaohan
Chen
Youwen Zhang
Youwen
Zhang
Ruiqi Xie
Ruiqi
Xie
Rui Ma
Rui
Ma
Dien Li
Dien
Li
Huazhong Li
Huazhong
Li
Xiyun Guan
Xiyun
Guan
Peptide-Mediated Nanopore Detection of Uranyl Ions
in Aqueous Media
American Chemical Society
2017
Peptide-Mediated Nanopore Detection
peptide events
water samples
micromolar concentrations
monitoring
uranyl ions
Aqueous Media Uranium
nanomolar concentration
peptide probe
Uranyl Ions
uranium production activity
label-free method
detection
UO
2017-05-04 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Peptide-Mediated_Nanopore_Detection_of_Uranyl_Ions_in_Aqueous_Media/4996700
Uranium
is one of the most common radioactive contaminants in the
environment. As a major nuclear material in production, environmental
samples (like soil and groundwater) can provide signatures on uranium
production activity inside the facility. Thus, developing a new and
portable analytical technology for uranium in aqueous media is significant
not only for environmental monitoring, but also for nonproliferation.
In this work, a label-free method for the detection of uranyl (UO<sub>2</sub><sup>2+</sup>) ions is developed by monitoring the translocation
of a peptide probe in a nanopore. Based on the difference in the number
of peptide events in the absence and presence of uranyl ions, nanomolar
concentration of UO<sub>2</sub><sup>2+</sup> ions could be detected
in minutes. The method is highly selective; micromolar concentrations
of Cd<sup>2+</sup>, Cu<sup>2+</sup>, Zn<sup>2+</sup>, Ni<sup>2+</sup>, Pb<sup>2+</sup>, Hg<sup>2+</sup>, Th<sup>4+</sup>, Mg<sup>2+</sup>, and Ca<sup>2+</sup> would not interfere with the detection of UO<sub>2</sub><sup>2+</sup> ions. In addition, simulated water samples were
successfully analyzed.