Natural Products of <i>Picea</i> Endophytes
from the Acadian Forest
David
R. McMullin
Blake D. Green
Natasha C. Prince
Joey B. Tanney
J. David Miller
10.1021/acs.jnatprod.6b01157.s002
https://acs.figshare.com/articles/journal_contribution/Natural_Products_of_i_Picea_i_Endophytes_from_the_Acadian_Forest/4865309
Endophytes of healthy needles were
collected from <i>Picea
rubens</i> (red spruce) and <i>P. mariana</i> (black
spruce) in a survey of southeastern New Brunswick, Canada. Four endophyte
strains were selected for further investigation based on the production
of biologically active extracts from culture filtrates during screening
as well as phylogenetic relationship to species known to produce natural
products or taxonomic novelty. A novel endophyte within the family
Rhytismataceae produced two new dihydropyrones (<b>1</b> and <b>2</b>) as major metabolites together with phthalides (<b>3</b> and <b>4</b>), isocoumarins (<b>5</b> and <b>6</b>), and tyrosol (<b>7</b>). <i>Lachnum</i> cf. <i>pygmaeum</i> synthesized a new chlorinated <i>para</i>-quinone, chloromycorrhizinone A (<b>8</b>), and the nematicidal
compounds (1′<i>Z</i>)-dechloromycorrhizin A (<b>9</b>), mycorrhizin A (<b>10</b>), and chloromycorrhizin
A (<b>11</b>). A new isocoumarin (<b>12</b>) and four
related structures (<b>13</b>–<b>16</b>) were isolated
from an undescribed taxon in the Mycosphaerellaceae. The known antifungal
metabolites cryptosporiopsin (<b>17</b>), 5-hydroxycryptosporiopsin
(<b>18</b>), (+)-cryptosporiopsinol (<b>19</b>), and mellein
(<b>20</b>) were produced by <i>Pezicula sporulosa</i>. Phylogenetically diverse conifer endophytes from the Acadian forest
continue to be a productive source of new biologically active natural
products.
2017-04-11 16:21:45
Lachnum cf
P . mariana
Acadian forest
culture filtrates
antifungal metabolites cryptosporiopsin
family Rhytismataceae
phylogenetic relationship
Natural Products
conifer endophytes
isocoumarin
chlorinated para
Pezicula sporulosa
Acadian Forest Endophytes
endophyte strains
Picea Endophytes
Picea rubens
New Brunswick
taxonomic novelty
. mariana
undescribed taxon
novel endophyte