10.1021/acs.nanolett.7b00814.s001
Davide Mariottini
Davide
Mariottini
Andrea Idili
Andrea
Idili
Alexis Vallée-Bélisle
Alexis
Vallée-Bélisle
Kevin W. Plaxco
Kevin W.
Plaxco
Francesco Ricci
Francesco
Ricci
A DNA Nanodevice That Loads and Releases a Cargo with
Hemoglobin-Like Allosteric Control and Cooperativity
American Chemical Society
2017
hemoglobin
binding sites
control mechanisms
oxygen-carrying activity
DNA Nanodevice
DNA-based nanodevice
Hemoglobin-Like Allosteric Control
DNA nanodevice
concentration ranges
nature-inspired control mechanisms
allosteric effectors
2017-04-07 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/A_DNA_Nanodevice_That_Loads_and_Releases_a_Cargo_with_Hemoglobin-Like_Allosteric_Control_and_Cooperativity/4865303
Here
we report the rational design of a synthetic molecular nanodevice
that is directly inspired from hemoglobin, a highly evolved protein
whose oxygen-carrying activity is finely regulated by a sophisticated
network of control mechanisms. Inspired by the impressive performance
of hemoglobin we have designed and engineered in vitro a synthetic
DNA-based nanodevice containing up to four interacting binding sites
that, like hemoglobin, can load and release a cargo over narrow concentration
ranges, and whose affinity can be finely controlled via both allosteric
effectors and environmental cues like pH and temperature. As the first
example of a synthetic DNA nanodevice that undergoes a complex network
of nature-inspired control mechanisms, this represents an important
step toward the use of similar nanodevices for diagnostic and drug-delivery
applications.