%0 Journal Article
%A Zhang, Shouting
%A Zhang, Dongxu
%A Zhang, Xuehong
%A Shang, Denghui
%A Xue, Zhonghua
%A Shan, Duoliang
%A Lu, Xiaoquan
%D 2017
%T Ultratrace Naked-Eye Colorimetric Detection of Hg2+ in Wastewater and Serum Utilizing Mercury-Stimulated Peroxidase
Mimetic Activity of Reduced Graphene Oxide-PEI-Pd Nanohybrids
%U https://acs.figshare.com/articles/journal_contribution/Ultratrace_Naked-Eye_Colorimetric_Detection_of_Hg_sup_2_sup_in_Wastewater_and_Serum_Utilizing_Mercury-Stimulated_Peroxidase_Mimetic_Activity_of_Reduced_Graphene_Oxide-PEI-Pd_Nanohybrids/4699156
%R 10.1021/acs.analchem.6b04805.s002
%2 https://acs.figshare.com/ndownloader/files/7672138
%K naked-eye-based colorimetric assay
%K ultratrace naked-eye colorimetric detection
%K solution
%K ultralow detection limit
%K Serum Utilizing Mercury-Stimulated Peroxidase Mimetic Activity
%K Hg
%K spectroscopic detection method
%K TMB
%K ddH 2 O
%K nanohybrid
%K strategy
%K Ultratrace Naked-Eye Colorimetric Detection
%K Reduced Graphene Oxide-PEI-Pd Nanohybrids Herein
%K nM
%K serum samples
%K peroxidase
%K absorption spectroscopic method
%K rGO
%K wastewater
%X Herein, we developed
a general strategy for rapid, highly selective,
and ultratrace naked-eye colorimetric detection of Hg2+ in aqueous solutions. Two dimensional rGO/PEI/Pd nanohybrids, where
rGO, PEI, and Pd were referred to as reduced graphene oxide, polyethylenimine,
and Pd nanoparticles, respectively, were synthesized and used as mimetic
peroxidase for selective and ultrasensitive detection of Hg2+ in water and human serum samples. In the presence of mercury ions,
the peroxidase mimetic activity of rGO/PEI/Pd nanohybrids was found
to be stimulated and enhanced significantly, which promoted the effective
oxidation and color change of 3,3′,5,5′-tetramethylbenzidine
(TMB) in solution to dark blue that was detected by the naked-eye
and the absorption spectroscopic method. The proposed sensing strategy
coupled with spectroscopic detection method showed an ultralow detection
limit of 0.39 nM for Hg2+ in ddH2O and ∼1
nM in wastewater as well as serum samples, respectively. On the basis
of the colorimetric assay, a minimum concentration of ∼10 nM
for Hg2+ in wastewater and human serum can be detected
with the naked-eye. The naked-eye-based colorimetric assay for sensitive
and selective detection of mercury is expected to hold huge potentials
in applications such as environmental monitoring, clinical diagnosis,
and pharmaceutical analysis.
%I ACS Publications