10.1021/acs.analchem.6b04805.s002
Shouting Zhang
Shouting
Zhang
Dongxu Zhang
Dongxu
Zhang
Xuehong Zhang
Xuehong
Zhang
Denghui Shang
Denghui
Shang
Zhonghua Xue
Zhonghua
Xue
Duoliang Shan
Duoliang
Shan
Xiaoquan Lu
Xiaoquan
Lu
Ultratrace Naked-Eye Colorimetric Detection of Hg<sup>2+</sup> in Wastewater and Serum Utilizing Mercury-Stimulated Peroxidase
Mimetic Activity of Reduced Graphene Oxide-PEI-Pd Nanohybrids
American Chemical Society
2017
naked-eye-based colorimetric assay
ultratrace naked-eye colorimetric detection
solution
ultralow detection limit
Serum Utilizing Mercury-Stimulated Peroxidase Mimetic Activity
Hg
spectroscopic detection method
TMB
ddH 2 O
nanohybrid
strategy
Ultratrace Naked-Eye Colorimetric Detection
Reduced Graphene Oxide-PEI-Pd Nanohybrids Herein
nM
serum samples
peroxidase
absorption spectroscopic method
rGO
wastewater
2017-02-16 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Ultratrace_Naked-Eye_Colorimetric_Detection_of_Hg_sup_2_sup_in_Wastewater_and_Serum_Utilizing_Mercury-Stimulated_Peroxidase_Mimetic_Activity_of_Reduced_Graphene_Oxide-PEI-Pd_Nanohybrids/4699156
Herein, we developed
a general strategy for rapid, highly selective,
and ultratrace naked-eye colorimetric detection of Hg<sup>2+</sup> in aqueous solutions. Two dimensional rGO/PEI/Pd nanohybrids, where
rGO, PEI, and Pd were referred to as reduced graphene oxide, polyethylenimine,
and Pd nanoparticles, respectively, were synthesized and used as mimetic
peroxidase for selective and ultrasensitive detection of Hg<sup>2+</sup> in water and human serum samples. In the presence of mercury ions,
the peroxidase mimetic activity of rGO/PEI/Pd nanohybrids was found
to be stimulated and enhanced significantly, which promoted the effective
oxidation and color change of 3,3′,5,5′-tetramethylbenzidine
(TMB) in solution to dark blue that was detected by the naked-eye
and the absorption spectroscopic method. The proposed sensing strategy
coupled with spectroscopic detection method showed an ultralow detection
limit of 0.39 nM for Hg<sup>2+</sup> in ddH<sub>2</sub>O and ∼1
nM in wastewater as well as serum samples, respectively. On the basis
of the colorimetric assay, a minimum concentration of ∼10 nM
for Hg<sup>2+</sup> in wastewater and human serum can be detected
with the naked-eye. The naked-eye-based colorimetric assay for sensitive
and selective detection of mercury is expected to hold huge potentials
in applications such as environmental monitoring, clinical diagnosis,
and pharmaceutical analysis.