Multifunctional
Nanoparticles by Coordinative Self-Assembly
of His-Tagged Units with Metal–Organic Frameworks
Ruth Röder
Tobias Preiß
Patrick Hirschle
Benjamin Steinborn
Andreas Zimpel
Miriam Höhn
Joachim O. Rädler
Thomas Bein
Ernst Wagner
Stefan Wuttke
Ulrich Lächelt
10.1021/jacs.6b11934.s002
https://acs.figshare.com/articles/media/Multifunctional_Nanoparticles_by_Coordinative_Self-Assembly_of_His-Tagged_Units_with_Metal_Organic_Frameworks/4622299
Self-assembly of
individual units into multicomponent complexes
is a powerful approach for the generation of functional superstructures.
We present the coordinative interaction of oligohistidine-tags (His-tags)
with metal–organic framework nanoparticles (MOF NPs). By this
novel concept, different molecular units can be anchored on the outer
surface of MOF NPs in a self-assembly process generating multifunctional
nanosystems. The article focuses on two main objectives: first, the
detailed investigation of the assembly process and fundamental establishment
of the novel functionalization concept; and second, its subsequent
use for the development of biomacromolecule (e.g., peptides and proteins)
delivery vehicles. Three exemplary MOF structures, MIL-88A, HKUST-1,
and Zr-<i>fum</i>, based on different metal components,
were selected for the external binding of various His-tagged synthetic
peptides and recombinant or chemically H<sub>6</sub>-modified proteins.
Evidence for simultaneous assembly of different functional units with
Zr-<i>fum</i> MOF NPs as well as their successful transport
into living cells illustrate the promising potential of the self-assembly
approach for the generation of multifunctional NPs and future biological
applications. Taking the high number of possible MOF NPs and different
functional units into account, the reported functionalization approach
opens great flexibility for the targeted synthesis of multifunctional
NPs for specific purposes.
2017-01-11 00:00:00
fum MOF NPs
MIL
-88A
multifunctional NPs
approach
HKUST
MOF NPs
novel functionalization concept