Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped
Interferometric Scattering Microscopy
Daniel Cole
Gavin Young
Alexander Weigel
Aleksandar Sebesta
Philipp Kukura
10.1021/acsphotonics.6b00912.s003
https://acs.figshare.com/articles/media/Label-Free_Single-Molecule_Imaging_with_Numerical-Aperture-Shaped_Interferometric_Scattering_Microscopy/4596457
Our
ability to optically interrogate nanoscopic objects is controlled
by the difference between their extinction cross sections and the
diffraction-limited area to which light can be confined in the far
field. We show that a partially transmissive spatial mask placed near
the back focal plane of a high numerical aperture microscope objective
enhances the extinction contrast of a scatterer near an interface
by approximately <i>T</i><sup>–1/2</sup>, where <i>T</i> is the transmissivity of the mask. Numerical-aperture-based
differentiation of background from scattered light represents a general
approach to increasing extinction contrast and enables routine label-free
imaging down to the single-molecule level.
2017-01-18 00:00:00
Numerical-Aperture-Shaped Interferometric Scattering Microscopy
Label-Free Single-Molecule Imaging
aperture microscope objective
extinction contrast