10.1021/acs.analchem.6b04114.s001 Deyan Gong Deyan Gong Xiangtao Zhu Xiangtao Zhu Yuejun Tian Yuejun Tian Shi-Chong Han Shi-Chong Han Min Deng Min Deng Anam Iqbal Anam Iqbal Weisheng Liu Weisheng Liu Wenwu Qin Wenwu Qin Huichen Guo Huichen Guo A Phenylselenium-Substituted BODIPY Fluorescent Turn-off Probe for Fluorescence Imaging of Hydrogen Sulfide in Living Cells American Chemical Society 2017 intracellular H 2 S detection Living Cells Herein H 2 S detection color fluorescence intensity decrease fluorescence microscopy imaging sensor phenylselenide group substitution BODIPY H 2 S fluorescence emission intensity 2017-01-13 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/A_Phenylselenium-Substituted_BODIPY_Fluorescent_Turn-off_Probe_for_Fluorescence_Imaging_of_Hydrogen_Sulfide_in_Living_Cells/4585354 Herein a phenylselenium-substituted BODIPY (<b>1</b>) fluorescent turn-off sensor was developed for the purpose to achieve excellent selectivity and sensitivity for H<sub>2</sub>S detection based on the substitution reaction of the phenylselenide group at the 3-position with H<sub>2</sub>S. The excess addition of hydrogen sulfide promoted further substitution of the phenylselenide group at the 5-position of the probe and was accompanied by a further decrease in fluorescence emission intensity. Sensor <b>1</b> demonstrated remarkable performance with 49-fold red color fluorescence intensity decrease at longer excitation wavelength, a low detection limit (0.0025 μM), and specific fluorescent response toward H<sub>2</sub>S over anions, biothiols, and other amino acids in neutral media. It showed no obvious cell toxicity and good membrane permeability, which was well exploited for intracellular H<sub>2</sub>S detection and imaging through fluorescence microscopy imaging.