10.1021/acs.analchem.6b04114.s001
Deyan Gong
Deyan
Gong
Xiangtao Zhu
Xiangtao
Zhu
Yuejun Tian
Yuejun
Tian
Shi-Chong Han
Shi-Chong
Han
Min Deng
Min
Deng
Anam Iqbal
Anam
Iqbal
Weisheng Liu
Weisheng
Liu
Wenwu Qin
Wenwu
Qin
Huichen Guo
Huichen
Guo
A Phenylselenium-Substituted BODIPY Fluorescent Turn-off
Probe for Fluorescence Imaging of Hydrogen Sulfide in Living Cells
American Chemical Society
2017
intracellular H 2 S detection
Living Cells Herein
H 2 S detection
color fluorescence intensity decrease
fluorescence microscopy imaging
sensor
phenylselenide group
substitution
BODIPY
H 2 S
fluorescence emission intensity
2017-01-13 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/A_Phenylselenium-Substituted_BODIPY_Fluorescent_Turn-off_Probe_for_Fluorescence_Imaging_of_Hydrogen_Sulfide_in_Living_Cells/4585354
Herein
a phenylselenium-substituted BODIPY (<b>1</b>) fluorescent
turn-off sensor was developed for the purpose to achieve excellent
selectivity and sensitivity for H<sub>2</sub>S detection based on
the substitution reaction of the phenylselenide group at the 3-position
with H<sub>2</sub>S. The excess addition of hydrogen sulfide promoted
further substitution of the phenylselenide group at the 5-position
of the probe and was accompanied by a further decrease in fluorescence
emission intensity. Sensor <b>1</b> demonstrated remarkable
performance with 49-fold red color fluorescence intensity decrease
at longer excitation wavelength, a low detection limit (0.0025 μM),
and specific fluorescent response toward H<sub>2</sub>S over anions,
biothiols, and other amino acids in neutral media. It showed no obvious
cell toxicity and good membrane permeability, which was well exploited
for intracellular H<sub>2</sub>S detection and imaging through fluorescence
microscopy imaging.