Microfluidic Pipette Tip for High-Purity and High-Throughput Blood Plasma Separation from Whole Blood Byeongyeon Kim Sein Oh Dongwon You Sungyoung Choi 10.1021/acs.analchem.6b04587.s001 https://acs.figshare.com/articles/journal_contribution/Microfluidic_Pipette_Tip_for_High-Purity_and_High-Throughput_Blood_Plasma_Separation_from_Whole_Blood/4541335 Blood plasma separation from whole blood is often limited by numerous blood cells which can compromise separation processes and thus deteriorate separation performance such as purity and throughput. To address this challenge, we present a microfluidic pipet tip composed of slant array ridges that enable autonomous blood cell focusing without significant deviation as well as facilitating a high degree of parallelization without compromising separation purity. With these advantages, we achieved high-purity (99.88%) and high-throughput (904.3 μL min<sup>–1</sup>) plasma separation from whole blood. In combination with a smart pipet, we successfully demonstrated rapid, inexpensive, and equipment-free blood plasma preparation for pretransfusion testing. 2017-01-11 18:53:26 pretransfusion testing separation processes separation purity blood cells separation performance equipment-free blood plasma preparation Whole Blood Blood plasma separation High-Throughput Blood Plasma Separation plasma separation blood cell Microfluidic Pipette Tip microfluidic pipet tip slant array ridges