Stiller, Christiane Krüger, Dennis M. Brauckhoff, Nicolas Schmidt, Marcel Janning, Petra Salamon, Hazem Grossmann, Tom N. Translocation of an Intracellular Protein via Peptide-Directed Ligation Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number of appropriate ligands and the lack of design principles. Addressing these limitations, we report a proximity-induced labeling system which applies a moderate affinity peptide ligand. The design process was structure-guided and supported by molecular dynamics simulations. We show that selective protein labeling can be performed inside living cells enabling the subcellular translocation of a protein via ligand-directed chemistry for the first time. proximity-induced reactions;chemical transformations;protein;design process;Intracellular Protein;subcellular translocation;design principles;ligand-directed chemistry;dynamics simulations;reactant concentrations;post-translational modification;Peptide-Directed Ligation Ligand-directed reactions;affinity peptide ligand 2016-12-21
    https://acs.figshare.com/articles/journal_contribution/Translocation_of_an_Intracellular_Protein_via_Peptide-Directed_Ligation/4509539
10.1021/acschembio.6b01013.s001