10.1021/acs.jmedchem.6b01011.s002 Yibo Qiu Yibo Qiu Misako Taichi Misako Taichi Na Wei Na Wei Huan Yang Huan Yang Kathy Qian Luo Kathy Qian Luo James P. Tam James P. Tam An Orally Active Bradykinin B<sub>1</sub> Receptor Antagonist Engineered as a Bifunctional Chimera of Sunflower Trypsin Inhibitor American Chemical Society 2016 serum half-life Sunflower Trypsin Inhibitor 6 h fusion cyclic peptide bradykinin receptor antagonist peptide sunflower trypsin inhibitor -1. Bifunctional Chimera plate assay metabolically peptide TIBA Bradykinin B 1 Receptor Antagonist Engineered animal pain model chimera TIBA 2016-12-05 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/An_Orally_Active_Bradykinin_B_sub_1_sub_Receptor_Antagonist_Engineered_as_a_Bifunctional_Chimera_of_Sunflower_Trypsin_Inhibitor/4451189 An orally active and metabolically stable peptide TIBA was successfully engineered as a chimera by fusing an analgesic bradykinin receptor antagonist peptide and the trypsin inhibitory loop of sunflower trypsin inhibitor-1. As a fusion cyclic peptide, the metabolically labile analgesic peptide is protected from degradation by exopeptidases as well as the endopeptidases, and its serum half-life extended from <5 min to >6 h as a chimera. Moreover, the chimera TIBA was also found to be orally active in an animal pain model using a hot plate assay.