Poly(glycerol) Used for Constructing Mixed Polymeric
Micelles as <i>T</i><sub>1</sub> MRI Contrast Agent for
Tumor-Targeted Imaging
Yi Cao
Min Liu
Kunchi Zhang
Guangyue Zu
Ye Kuang
Xiaoyan Tong
Dangsheng Xiong
Renjun Pei
10.1021/acs.biomac.6b01437.s001
https://acs.figshare.com/articles/journal_contribution/Poly_glycerol_Used_for_Constructing_Mixed_Polymeric_Micelles_as_i_T_i_sub_1_sub_MRI_Contrast_Agent_for_Tumor-Targeted_Imaging/4282169
There
was much interest in the development of nanoscale delivery
vehicles based on polymeric micelles to realize the diagnostic and
therapeutic applications in biomedicine. Here, with the purpose of
constructing a micellar magnetic resonance imaging (MRI) contrast
agent (CA) with well biocompatibility and targeting specificity, two
types of amphiphilic diblock polymers, mPEG–PG(DOTA(Gd))-<i>b</i>-PCL and FA-PEG-<i>b</i>-PCL, were synthesized
to form mixed micelles by coassembly. The nanostructure of the resulting
micellar system consisted of poly(caprolactone) (PCL) as core and
poly(glycerol) (PG) and poly(ethylene glycol) (PEG) as shell, simultaneously
modified with DOTA(Gd) chelates and folic acid (FA), which afforded
functions of MRI contrast enhancement and tumor targeting. The mixed
micelles in aqueous solution presented a hydrodynamic diameter of
about 85 nm. Additionally, this mixed micelles exhibited higher <i>r</i><sub>1</sub> relaxivity (14.01 mM<sup>–1</sup> S<sup>1–</sup>) compared with commercial Magnevist (3.95 mM<sup>–1</sup> S<sup>1–</sup>) and showed negligible cytotoxicity
estimated by WST assay. In vitro and in vivo MRI experiments revealed
excellent targeting specificity to tumor cells and tissue. Furthermore,
considerably enhanced signal intensity and prominent positive contrast
effect were achieved at tumor region after tumor-bearing mice were
intravenously injected with the mixed micelles. These preliminary
results indicated the potential of the mixed micelle as <i>T</i><sub>1</sub> MRI CA for tumor-targeted imaging.
2016-11-21 00:00:00
signal intensity
PG
vivo MRI experiments
WST assay
amphiphilic diblock polymers
PCL
T 1 MRI CA
micelle
DOTA
MRI contrast enhancement
tumor-targeted imaging
folic acid
Tumor-Targeted Imaging
FA
PEG
Constructing Mixed Polymeric Micelles
hydrodynamic diameter
85 nm
tumor cells
contrast agent
nanoscale delivery vehicles
micellar system
tumor-bearing mice
tumor region
FA-PEG
T 1 MRI Contrast Agent
resonance imaging
contrast effect