10.1021/acs.jafc.6b04435.s001 Christin Fischer Christin Fischer Constanze Kallinich Constanze Kallinich Sven Klockmann Sven Klockmann Jil Schrader Jil Schrader Markus Fischer Markus Fischer Automated Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer-Linked Magnetic Particles American Chemical Society 2016 antibiotic sulfanilamide fluorescence assay cocoa milk drink Automated Enrichment Enrichment factors DNA aptamers 30 min separator increases milk matrices enrichment approach Milk Matrices dissociation constants LC-MS novel implementation 2016-11-09 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Automated_Enrichment_of_Sulfanilamide_in_Milk_Matrices_by_Utilization_of_Aptamer-Linked_Magnetic_Particles/4264388 The present work demonstrates the first automated enrichment approach for antibiotics in milk using specific DNA aptamers. First, aptamers toward the antibiotic sulfanilamide were selected and characterized regarding their dissociation constants and specificity toward relevant antibiotics via fluorescence assay and LC-MS/MS detection. The performed enrichment was automated using the KingFisherDuo and compared to a manual approach. Verifying the functionality, trapping was realized in different milk matrices: (i) 0.3% fat milk, (ii) 1.5% fat milk, (iii) 3.5% fat milk, and (iv) 0.3% fat cocoa milk drink. Enrichment factors up to 8-fold could be achieved. Furthermore, it could be shown that novel implementation of a magnetic separator increases the reproducibility and reduces the hands-on time from approximately half a day to 30 min.