10.1021/acs.jafc.6b04435.s001
Christin Fischer
Christin
Fischer
Constanze Kallinich
Constanze
Kallinich
Sven Klockmann
Sven
Klockmann
Jil Schrader
Jil
Schrader
Markus Fischer
Markus
Fischer
Automated Enrichment of Sulfanilamide in Milk Matrices
by Utilization of Aptamer-Linked Magnetic Particles
American Chemical Society
2016
antibiotic sulfanilamide
fluorescence assay
cocoa milk drink
Automated Enrichment
Enrichment factors
DNA aptamers
30 min
separator increases
milk matrices
enrichment approach
Milk Matrices
dissociation constants
LC-MS
novel implementation
2016-11-09 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Automated_Enrichment_of_Sulfanilamide_in_Milk_Matrices_by_Utilization_of_Aptamer-Linked_Magnetic_Particles/4264388
The
present work demonstrates the first automated enrichment approach
for antibiotics in milk using specific DNA aptamers. First, aptamers
toward the antibiotic sulfanilamide were selected and characterized
regarding their dissociation constants and specificity toward relevant
antibiotics via fluorescence assay and LC-MS/MS detection. The performed
enrichment was automated using the KingFisherDuo and compared to a
manual approach. Verifying the functionality, trapping was realized
in different milk matrices: (i) 0.3% fat milk, (ii) 1.5% fat milk,
(iii) 3.5% fat milk, and (iv) 0.3% fat cocoa milk drink. Enrichment
factors up to 8-fold could be achieved. Furthermore, it could be shown
that novel implementation of a magnetic separator increases the reproducibility
and reduces the hands-on time from approximately half a day to 30
min.