10.1021/acsami.6b11287.s001
Bin Li
Bin
Li
Kaixuan Ren
Kaixuan
Ren
Yupeng Wang
Yupeng
Wang
Yanxin Qi
Yanxin
Qi
Xuesi Chen
Xuesi
Chen
Yubin Huang
Yubin
Huang
Protein-Cross-Linked
Hydrogels with Tailored Swelling and Bioactivity Performance: A Comparative
Study
American Chemical Society
2016
Bioactivity Performance
Michael addition reaction
stimuli-responsive hydrogel
macroscopic hydrogel
DTT
Protein-Cross-Linked Hydrogels
multimeric proteins
Results show
4- arm PEG-acrylate
Comparative Study
bioengineering applications
multisite cross-linkers
hydrogel cross-linked
protein-based hydrogels
protein dissociation
structure change
protein-based hydrogel
thiolation agent
2016-11-02 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Protein-Cross-Linked_Hydrogels_with_Tailored_Swelling_and_Bioactivity_Performance_A_Comparative_Study/4214466
The
design of protein-based hydrogels that include biological activity
independent of structural functionality is desirable for many bioengineering
applications. Here a general route for construction of protein-based
hydrogel is proposed by pretreatment of protein with thiolation agent
and succeeding conjugation with 4-arm PEG-acrylate via Michael addition
reaction. Different swelling behaviors responding to temperature and
ions are comparatively studied for hydrogel cross-linked with hemoglobin
(multimeric protein), albumin (monomeric protein), and dithiothreitol
(DTT, small molecule). Meanwhile, the microscopic structure change
is studied to correlate with the macroscopic hydrogel swelling behavior.
Results show that proteins, which function as multisite cross-linkers,
affect the gel swelling behaviors, and the effect is more profound
for multimeric proteins when exposed to stimulus for protein dissociation.
Moreover, the catalytic activity derived from hemoglobin is also preserved
in the hydrogel, as demonstrated by the successfully synthesis of
the colored product. By taking advantage of each particular protein,
a broad range of functional materials can be expected for potential
biomedical applications, such as stimuli-responsive hydrogel and immobilized
enzyme.