10.1021/acsnano.6b06411.s003
Filippo Bertoli
Filippo
Bertoli
David Garry
David
Garry
Marco
P. Monopoli
Marco
P.
Monopoli
Anna Salvati
Anna
Salvati
Kenneth A. Dawson
Kenneth A.
Dawson
The
Intracellular Destiny of the Protein Corona: A
Study on its Cellular Internalization and Evolution
American Chemical Society
2016
extracellular medium
extracellular corona
Intracellular Destiny
nanoparticles travel
Protein Corona
protein corona
biomolecular corona
intracellular processing
Cellular Internalization
endolysosomal pathway
serum proteins
membrane interactions
nanoparticle surface
intracellular evolution
organelle separation
2016-10-31 00:00:00
Media
https://acs.figshare.com/articles/media/The_Intracellular_Destiny_of_the_Protein_Corona_A_Study_on_its_Cellular_Internalization_and_Evolution/4195656
It
has been well established that the early stages of nanoparticle–cell
interactions are governed, at least in part, by the layer of proteins
and other biomolecules adsorbed and slowly exchanged with the surrounding
biological media (biomolecular corona). Subsequent to membrane interactions,
nanoparticles are typically internalized into the cell and trafficked
along defined pathways such as, in many cases, the endolysosomal pathway.
Indeed, if the original corona is partially retained on the nanoparticle
surface, the biomolecules in this layer may play an important role
in determining subsequent cellular processing. In this work, using
a combination of organelle separation and fluorescence labeling of
the initial extracellular corona, we clarify its intracellular evolution
as nanoparticles travel within the cell. We show that specific proteins
present in the original protein corona are retained on the nanoparticles
until they accumulate in lysosomes, and, once there, they are degraded.
We also report on how different bare surfaces (amino and carboxyl
modified) affect the details of this evolution. One overarching discovery
is that the same serum proteins can exhibit different intracellular
processing when carried inside cells by nanoparticles, as components
of their corona, compared to what is observed when they are transported
freely from the extracellular medium.