10.1021/acs.biomac.6b01068.s002
David
W. Howell
David
W.
Howell
Camille L. Duran
Camille L.
Duran
Shang-Pu Tsai
Shang-Pu
Tsai
Sarah E. Bondos
Sarah E.
Bondos
Kayla J. Bayless
Kayla
J. Bayless
Functionalization of Ultrabithorax Materials with
Vascular Endothelial Growth Factor Enhances Angiogenic Activity
American Chemical Society
2016
vessel formation
growth factors
gene encoding Vascular Endothelial Growth Factor
VEGF-Ubx materials
CAM
fusion proteins
Ubx materials
cell migration
pattern angiogenesis
Ultrabithorax Materials
chicken embryo chorioallantoic membrane
outgrowing sprouts
cell survival
VEGF-Ubx fibers
tissue engineering scaffolds
aortic ring assay
angiogenic growth factors
Vascular Endothelial Growth Factor Enhances Angiogenic Activity
Current approaches
2016-10-07 00:00:00
Media
https://acs.figshare.com/articles/media/Functionalization_of_Ultrabithorax_Materials_with_Vascular_Endothelial_Growth_Factor_Enhances_Angiogenic_Activity/4040622
Successful
design of tissue engineering scaffolds must include
the ability to stimulate vascular development by incorporating angiogenic
growth factors. Current approaches can allow diffusion of growth factors,
incorporate active factors randomly, or can leave residual toxins.
We addressed these problems by genetically fusing the gene encoding
Vascular Endothelial Growth Factor (VEGF) with the Ultrabithorax (Ubx)
gene to produce fusion proteins capable of self-assembly into materials.
We demonstrate that VEGF-Ubx materials enhance human endothelial cell
migration, prolong cell survival, and dose-dependently activate the
VEGF signaling pathway. VEGF-Ubx fibers attract outgrowing sprouts
in an aortic ring assay and induce vessel formation in a chicken embryo
chorioallantoic membrane (CAM) assay. Collectively, these results
demonstrate that the activity of VEGF remains intact in Ubx materials.
This approach could provide an inexpensive and facile mechanism to
stimulate and pattern angiogenesis.