Yang, Xu Gelfanov, Vasily Liu, Fa DiMarchi, Richard Synthetic Route to Human Relaxin‑2 via Iodine-Free Sequential Disulfide Bond Formation A new synthetic route to human relaxin-2 has been established through a sequential disulfide bond formation process in the absence of iodine. It is enabled by a combination of cysteine protection with penicillin G acylase-labile Phacm and a newly identified thiol activator bis­(5-(2-methoxyethoxy)-2-pyrimidinyl disulfide. The long-standing challenges in relaxin B-chain assembly and its poor solubility have been solved by the insertion of two isoacyl dipeptide segments. The overall yield was 25% from the B chain and 5.8% from the B-chain starting resin. Relaxin;insertion;relaxin B-chain assembly;iodine;Synthetic Route;absence;sequential disulfide bond formation process;thiol;B chain;penicillin G acylase-labile Phacm;combination;solubility;activator;Iodine-Free Sequential Disulfide Bond Formation;relaxin -2;isoacyl dipeptide segments;resin;cysteine protection;Human;challenge 2016-10-18
    https://acs.figshare.com/articles/journal_contribution/Synthetic_Route_to_Human_Relaxin_2_via_Iodine-Free_Sequential_Disulfide_Bond_Formation/4039806
10.1021/acs.orglett.6b02751.s001