Improving the Intracellular Drug Concentration in Lung Cancer Treatment through the Codelivery of Doxorubicin and miR-519c Mediated by Porous PLGA Microparticle Di Wu Chenhui Wang Jiebing Yang Hao Wang Haobo Han Aijun Zhang Yan Yang Quanshun Li 10.1021/acs.molpharmaceut.6b00702.s001 https://acs.figshare.com/articles/journal_contribution/Improving_the_Intracellular_Drug_Concentration_in_Lung_Cancer_Treatment_through_the_Codelivery_of_Doxorubicin_and_miR-519c_Mediated_by_Porous_PLGA_Microparticle/3993720 Porous PLGA microparticle for the coencapsulation of doxorubicin and miR-519c was successfully constructed through the water–oil–water emulsion solvent evaporation method, using ammonium bicarbonate as a porogen. It has been characterized with high porous surface, adaptive aerodynamic diameter (<10 μm), favorable drug loading, and sustained release profile. The release supernatant exhibited a higher inhibition of cell proliferation than those from porous PLGA microparticles harboring a single component (doxorubicin or miR-519c), attributing to the enhanced induction of cell apoptosis and cell cycle arrest at S phase. Finally, the improved intracellular concentration of doxorubicin was elucidated by flow cytometry and liquid chromatography with tandem mass spectrometry, owing to the knockdown of drug transporter ABCG2 by miR-519c. Overall, the porous PLGA microparticle combining chemotherapy and gene therapy could facilitate the antitumor efficacy and reduce the side effects, and thus, it is potential to be used as a sustained release system for lung cancer treatment via pulmonary administration. 2016-09-29 00:00:00 tandem mass spectrometry release cell cycle arrest drug transporter ABCG 2 lung cancer treatment Lung Cancer Treatment Porous PLGA Microparticle Porous PLGA microparticle doxorubicin miR -519c Intracellular Drug Concentration miR -519c Mediated