Characterization of Lipid-Based Hexosomes as Versatile
Vaccine Carriers
Letícia Rodrigues
Konstantinos Kyriakos
Fabian Schneider
Hendrik Dietz
Gerhard Winter
Christine
M. Papadakis
Madlen Hubert
10.1021/acs.molpharmaceut.6b00716.s001
https://acs.figshare.com/articles/journal_contribution/Characterization_of_Lipid-Based_Hexosomes_as_Versatile_Vaccine_Carriers/3860370
Subunit vaccines
typically show insufficient immunogenicity. To
address this issue, we developed a novel self-adjuvanting particulate
carrier system based upon the lipids phytantriol (Phy) and mannide
monooleate (MaMo). Phy is a lipid known to form nonlamellar phases
in fully hydrated systems, whereas MaMo has been found to promote
immune responses in emulsion form. A bulk phase composition of Phy/MaMo
(14 wt %) showed hexagonal (HII) phase behavior over a practical temperature
range (including room and body temperature), and was therefore used
for particle development. Hexosomes stabilized with different concentrations
of either poloxamer 407, Myrj 59, or Pluronic F108 were successfully
prepared. To demonstrate the versatile nature of these systems, the
particles were further modified with either positively or negatively
charged lipids and loaded with model antigens, while maintaining the
HII structure. These hexosomes are structurally robust and amenable
to customization, rendering them suitable as antigen delivery carriers.
2016-09-08 00:00:00
MaMo
Versatile Vaccine Carriers Subunit vaccines
mannide monooleate
emulsion form
novel self-adjuvanting
antigen delivery carriers
Phy
Lipid-Based Hexosomes
bulk phase composition
Pluronic F 108
lipids phytantriol
HII structure
model antigens
carrier system
phase behavior
form nonlamellar phases
temperature range
particle development