10.1021/acssensors.6b00379.s002
Bo Tian
Bo
Tian
Jing Ma
Jing
Ma
Teresa Zardán Gómez de la Torre
Teresa
Zardán Gómez de la Torre
Ádám Bálint
Ádám
Bálint
Marco Donolato
Marco
Donolato
Mikkel
Fougt Hansen
Mikkel
Fougt
Hansen
Peter Svedlindh
Peter
Svedlindh
Mattias Strömberg
Mattias
Strömberg
Rapid Newcastle Disease Virus Detection Based on Loop-Mediated
Isothermal Amplification and Optomagnetic Readout
American Chemical Society
2016
LAMP primers
RT-PCR
target sequence
user-friendly readout methods
Newcastle disease virus RNA
PCR
detection
MNP
assay time
amplification schemes
Loop-Mediated Isothermal Amplification
Optomagnetic Readout Rapid
10 aM
transcription LAMP
hydrodynamic size
DNA
Rapid Newcastle Disease Virus Detection
tissue specimens
30 min
out-of-lab settings
RT-LAMP
Biotinylated amplicons
optomagnetic nanoparticle-based readout system
LAMP target sequence
amplification efficiency
optomagnetic readout system
2016-09-19 00:00:00
Dataset
https://acs.figshare.com/articles/dataset/Rapid_Newcastle_Disease_Virus_Detection_Based_on_Loop-Mediated_Isothermal_Amplification_and_Optomagnetic_Readout/3859536
Rapid and sensitive diagnostic methods
based on isothermal amplification
are ideal substitutes for PCR in out-of-lab settings. However, there
are bottlenecks in terms of establishing low-cost and user-friendly
readout methods for isothermal amplification schemes. Combining the
high amplification efficiency of loop-mediated isothermal amplification
(LAMP) with an optomagnetic nanoparticle-based readout system, we
demonstrate ultrasensitive and rapid detection of Newcastle disease
virus RNA. Biotinylated amplicons of LAMP and reverse transcription
LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles
(MNPs) resulting in a dramatical increase in the hydrodynamic size
of the MNPs. This increase was measured by an optomagnetic readout
system and provided quantitative information on the amount of LAMP
target sequence. Our assay resulted in a limit of detection of 10
aM of target sequence with a total assay time of 30 min. The assay
has also been tested on clinical samples (vaccine and tissue specimens)
with a performance comparable to real-time RT-PCR. By changing the
LAMP primers, this strategy can serve as a general method for the
detection of other DNA/RNA targets with high specificity and sensitivity.