%0 Journal Article
%A Qi, Meng
%A Zhang, Yin
%A Cao, Chaomin
%A Zhang, Mingxing
%A Liu, Shenghua
%A Liu, Guozhen
%D 2016
%T Decoration of Reduced
Graphene Oxide Nanosheets with
Aryldiazonium Salts and Gold Nanoparticles toward a Label-Free Amperometric
Immunosensor for Detecting Cytokine Tumor Necrosis Factor‑α
in Live Cells
%U https://acs.figshare.com/articles/journal_contribution/Decoration_of_Reduced_Graphene_Oxide_Nanosheets_with_Aryldiazonium_Salts_and_Gold_Nanoparticles_toward_a_Label-Free_Amperometric_Immunosensor_for_Detecting_Cytokine_Tumor_Necrosis_Factor_in_Live_Cells/3846564
%R 10.1021/acs.analchem.6b02353.s001
%2 https://acs.figshare.com/ndownloader/files/6014214
%K FT-IR
%K XPS
%K Reduced Graphene Oxide Nanosheets
%K Label-Free Amperometric Immunosensor
%K cytokine tumor necrosis factor-alpha
%K enzyme-linked immunosorbent assay
%K anti-TNF -α detection antibody
%K UV
%K BV -2 cells
%K graphene oxides nanocomposites
%K 4- aminophenyl phosphorylcholine
%K TEM
%K ELISA
%K TNF -α
%K surface
%K PPC
%K analyte TNF -α
%K form Ab 2
%K label-free electrochemical immunosensor
%K 50 mM phosphate buffer
%K X-ray photoelectron spectra
%X In this study, a label-free electrochemical
immunosensor was developed
for detection of cytokine tumor necrosis factor-alpha (TNF-α).
First, AuNPs loaded reduced graphene oxides nanocomposites (RGO-ph-AuNP)
were prepared, and then, a mixed layer of 4-carbxyphenyl and 4-aminophenyl
phosphorylcholine (PPC) was modified to the surface of AuNPs for the
subsequent modification of anti-TNF-α capture antibody (Ab1) to form the capture surface (Au-RGO-ph-AuNP-ph-PPC(-ph-COOH))
for the analyte TNF-α with the antifouling property. For reporting
the presence of analyte, the anti-TNF-α detection antibody (Ab2) was modified to the graphene oxides which have been modified
with the 4-ferrocenylaniline through diazonium chemistry to form Ab2-GO-ph-Fc. Then, a sandwich assay was formed on gold surfaces
for the quantitative detection of TNF-α based on the electrochemical
signal of ferrocene. X-ray photoelectron spectra (XPS), transmission
electron microscopy (TEM), Fourier transform infrared spectroscopy
(FT-IR), UV–vis, and electrochemistry were used for characterization
of the stepwise fabrications on the interface. The prepared electrochemical
immunosensor was successfully used for the detection of TNF-α
over the range of 0.1–150 pg mL–1. The lowest
detection limit of this immunosensor is 0.1 pg mL–1 TNF-α in 50 mM phosphate buffer at pH 7.0. The fabricated
immunosensor provided high selectivity and stability and can be used
to detect TNF-α secreted by live BV-2 cells with comparable
accuracy to enzyme-linked immunosorbent assay (ELISA) but with lower
limit of detection.
%I ACS Publications