Fischer, Carsten Rodríguez, Leticia Patallo, Eugenio P. Lipata, Fredilyn Braña, Alfredo F. Méndez, Carmen Salas, Jose A. Rohr, Jürgen Digitoxosyltetracenomycin C and Glucosyltetracenomycin C, Two Novel Elloramycin Analogues Obtained by Exploring the Sugar Donor Substrate Specificity of Glycosyltransferase ElmGT Our explorations of glycosyltransferase ElmGT from <i>Streptomyces olivaceus </i>Tü 2353, which shows an interesting flexibility regarding its sugar donor substrate, were extended toward various previously unexplored sugar co-substrates. The studies revealed that ElmGT, which normally transfers l-rhamnose to 8-demethyltetracenomycin C as a crucial biosynthetic step in elloramycin biosynthesis, is also able to process an activated non-deoxygenated sugar, NDP-d-glucose, as well as NDP-l-digitoxose, which is the first example of an NDP-l-sugar co-substrate of ElmGT possessing an axial 3-OH group. The structures of the resulting novel elloramycin analogues of these experiments, 8-demethyl-8-l-digitoxosyltetracenomycin C (<b>4</b>) and 8-demethyl-8-d-glucosyltetracenomycin C (<b>7</b>), were elucidated mainly by <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy and by mass spectrometry. biosynthetic step;mass spectrometry;Novel Elloramycin Analogues Obtained;Sugar Donor Substrate Specificity;13 C NMR spectroscopy;Glucosyltetracenomycin C;sugar donor substrate;glycosyltransferase ElmGT;novel elloramycin analogues;demethyl;1 H;Digitoxosyltetracenomycin C;Glycosyltransferase ElmGT;OH;elloramycin biosynthesis 2002-09-05
    https://acs.figshare.com/articles/journal_contribution/Digitoxosyltetracenomycin_C_and_Glucosyltetracenomycin_C_Two_Novel_Elloramycin_Analogues_Obtained_by_Exploring_the_Sugar_Donor_Substrate_Specificity_of_Glycosyltransferase_ElmGT/3734313
10.1021/np020112z.s001