10.1021/jm0205604.s001
Daniel E. Levy
Daniel E.
Levy
Ming Bao
Ming
Bao
Diana B. Cherbavaz
Diana B.
Cherbavaz
James E. Tomlinson
James E.
Tomlinson
David M. Sedlock
David M.
Sedlock
Charles J. Homcy
Charles J.
Homcy
Robert M. Scarborough
Robert M.
Scarborough
Metal Coordination-Based Inhibitors of Adenylyl Cyclase: Novel Potent P-Site
Antagonists
American Chemical Society
2003
signal transduction inhibitors
adenylyl cyclase
SAR
ATP
purine binding site
adenine ring system
novel AC inhibitors
2003-04-19 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Metal_Coordination-Based_Inhibitors_of_Adenylyl_Cyclase_Novel_Potent_P-Site_Antagonists/3681309
The adenylyl cyclases (ACs) are a family of intracellular enzymes associated with signal
transduction by virtue of their ability to convert ATP to cAMP. The catalytic mechanism of
this transformation proceeds through initial binding of ATP to the so-called purine binding
site (P-site) of the enzyme followed by metal-mediated cyclization with loss of pyrophosphate.
Crystallographic analysis of ACs with known inhibitors reveals the presence of two metals in
the active site. Presently, nine isoforms of adenylyl cyclase are known, and unique isoform
combinations are expressed in a tissue-specific manner. The development of isoform-specific
inhibitors of adenylyl cyclase may prove to be a useful strategy toward the design of unique
signal transduction inhibitors. To develop novel AC inhibitors, we have chosen an approach to
inhibitor design utilizing an adenine ring system joined to a metal-coordinating hydroxamic
acid via various linkers. Previous work in our group has validated this approach and identified
novel inhibitors that possess an adenine ring joined to a metal-coordinating hydroxamic acid
through flexible acyclic linkers (Levy, D. E., et al. <i>Bioorg. Med. Chem. Lett.</i> <b>2002</b>, <i>12</i>, 3085−3088). Subsequent studies have focused on the introduction of conformational restrictions into
the tether of the inhibitors with the goal of increasing potency (Levy, D. E., et al. <i>Bioorg. Med.
Chem. Lett.</i> <b>2002</b>, <i>12</i>, 3089−3092). Building upon the favorable spatial positioning of the adenine
and hydroxamate groups coupled with potentially favorable entropic factors, the unit joining
the carbocycle to the hydroxamate was explored further and a stereochemical-based SAR was
elucidated, leading to a new series of highly potent AC inhibitors.