Inhibitors of Human Histone Deacetylase:  Synthesis and Enzyme and Cellular Activity of Straight Chain Hydroxamates Stacy W. Remiszewski Lidia C. Sambucetti Peter Atadja Kenneth W. Bair Wendy D. Cornell Michael A. Green Kobporn Lulu Howell Manfred Jung Paul Kwon Nancy Trogani Heather Walker 10.1021/jm015568c.s002 https://acs.figshare.com/articles/journal_contribution/Inhibitors_of_Human_Histone_Deacetylase_Synthesis_and_Enzyme_and_Cellular_Activity_of_Straight_Chain_Hydroxamates/3680886 Inhibitors of histone deacetylase (HDAC) have been shown to induce terminal differentiation of human tumor cell lines and to have antitumor effects in vivo. We have prepared analogues of suberoylanilide hydroxamic acid (SAHA) and trichostatin A and have evaluated them in a human HDAC enzyme inhibition assay, a p21<sup>waf1</sup> (p21) promoter assay, and in monolayer growth inhibition assays. One compound, 4-(dimethylamino)-<i>N</i>-[7-(hydroxyamino)-7-oxoheptyl]-benzamide, was found to affect the growth of a panel of eight human tumor cell lines differentially. 2002-01-17 00:00:00 histone deacetylase Straight Chain Hydroxamates Inhibitors monolayer growth inhibition assays p 21 waf 1 HDAC enzyme inhibition assay tumor cell lines terminal differentiation SAHA Cellular Activity tumor cell lines differentially suberoylanilide hydroxamic acid antitumor effects