10.1021/jf026075z.s001
Jared I. Loader
Jared I.
Loader
Alistair L. Wilkins
Alistair L.
Wilkins
Arne Flåøyen
Arne
Flåøyen
Evrind Ryste
Evrind
Ryste
Knut Hove
Knut
Hove
Ovine Metabolism of Lithogenic Sapogenins. Synthesis of
[2,2,4,4-<sup>2</sup>H<sub>4</sub>]Sarsasapogenone, [2,2,4,4-<sup>2</sup>H<sub>4</sub>]Sarsasapogenin, and
[2,2,4,4-<sup>2</sup>H<sub>4</sub>]Episarsasapogenin and Evaluation of Deuterium
Retention in a Sheep-Dosing Trial
American Chemical Society
2003
mixture
material
sarsasapogenin
dosing
sapogenin
sarsasapogenone
recovery
episarsasapogenin
3 b
deuterium
ovine heptagenous photosentization diseases
genins 2 b
2003-03-28 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Ovine_Metabolism_of_Lithogenic_Sapogenins_Synthesis_of_2_2_4_4-_sup_2_sup_H_sub_4_sub_Sarsasapogenone_2_2_4_4-_sup_2_sup_H_sub_4_sub_Sarsasapogenin_and_2_2_4_4-_sup_2_sup_H_sub_4_sub_Episarsasapogenin_and_Evaluation_of_Deuterium_Retention_in_a_Sheep-Dosin/3679302
The suitability of [2,2,4,4-<sup>2</sup>H<sub>4</sub>]sarsasapogenone (<b>1b</b>), [2,2,4,4-<sup>2</sup>H<sub>4</sub>]sarsasapogenin (<b>2b</b>), and [2,2,4,4-<sup>2</sup>H<sub>4</sub>]episarsasapogenin (<b>3b</b>) as isotopically labeled dosing substrates to determine the levels of free
and conjugated sapogenins present in feces from sheep grazing saponin-containing plants implicated
in the development of ovine heptagenous photosentization diseases was investigated. A 1:4 mixture
of [2,2,4,4-<sup>2</sup>H<sub>4</sub>]sarsasapogenin (<b>2b</b>) and [2,2,4,4-<sup>2</sup>H<sub>4</sub>]episarsasapogenin (<b>3b</b>), obtained by reduction
of [2,2,4,4-<sup>2</sup>H<sub>4</sub>]sarsasapogenone (<b>1b</b>), was found to retain 94% of incorporated deuterium, when dosed
to one sheep. The recovery of the dosed mixture of genins <b>2b</b> and <b>3b</b> was calculated to be 85%.
Considerable loss of deuterium and a lower recovery of genin material were observed when [2,2,4,4-<sup>2</sup>H<sub>4</sub>]sarsasapogenone (<b>1b</b>) was dosed.
Keywords: Deuterated sapogenins; dosing trial; sheep; fecal material; <i>Narthecium ossifragum</i>;
episarsasapogenin; sarsasapogenin; sarsasapogenone