10.1021/ja952014o.s002
Kevin Shreder
Kevin
Shreder
Anthony Harriman
Anthony
Harriman
Brent L. Iverson
Brent L.
Iverson
Molecular Recognition of a Monoclonal Antibody (AC1106)
Cross-Reactive for Derivatives of Ru(bpy)<sub>3</sub><sup>2+</sup> and Ru(phen)<sub>3</sub><sup>2+</sup>
American Chemical Society
1996
Ru
luminescence decay traces
methyl viologen moiety
Competition ELISA data
antibody binding site
AC 1106
methyl viologen
1996-04-03 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Molecular_Recognition_of_a_Monoclonal_Antibody_AC1106_Cross-Reactive_for_Derivatives_of_Ru_bpy_sub_3_sub_sup_2_sup_and_Ru_phen_sub_3_sub_sup_2_sup_/3656550
The characterization of a monoclonal antibody (AC1106) elicited
<i>via</i> immunization with a Co(dmbpy)(bpy)<sub>2</sub><sup>3+</sup>−methyl viologen hapten
(<b>1</b>) is described. AC1106 was found cross-reactive for a
variety of luminescent
ruthenium(II) metal complexes which served as useful probes to
investigate the molecular recognition properties of
this antibody. AC1106 was found to be specific for methylated
derivatives of Ru(bpy)<sub>3</sub><sup>2+</sup> and
Ru(phen)<sub>3</sub><sup>2+</sup> in the
order of Ru(dmbpy)<sub>3</sub><sup>2+</sup> >
Ru(dmbpy)(bpy)<sub>2</sub><sup>2+</sup> >
Ru(dmphen)<sub>3</sub><sup>2+</sup> >
Ru(bpy)<sub>3</sub><sup>2+</sup> ≫
Ru(phen)<sub>3</sub><sup>2+</sup>. The affinities
of
AC1106 for these metal complexes were found to range from ≥ 5 ×
10<sup>7</sup> to ≤ 1 × 10<sup>3</sup>
M<sup>-1</sup>. When bound (>98%)
by AC1106, the luminescence decay traces for the racemic
Ru(dmbpy)<sub>3</sub><sup>2+</sup> and
Ru(dmbpy)(bpy)<sub>2</sub><sup>2+</sup> gave a
satisfactory
fit to a single-exponential decay process. Furthermore,
D<sub>2</sub>O/H<sub>2</sub>O experiments with
Ru(dmbpy)<sub>3</sub><sup>2+</sup> indicate that
AC1106
protects approximately 70% of the antibody-bound
Ru(dmbpy)<sub>3</sub><sup>2+</sup> from excited state
deactivation by the solvent.
Competition ELISA data indicate that both the metal center and the
methyl viologen moiety present in a
Ru(bpy)<sub>3</sub><sup>2+</sup>−methyl viologen conjugate
([Ru(mv<sup>2+</sup>-bpy)(bpy)<sub>2</sub>]<sup>4+</sup>)
are important recognition elements for AC1106. Despite
the
apparent affinity of AC1106 for methyl viologen, no evidence for
simultaneous binding of methyl viologen and
Ru(dmbpy)(bpy)<sub>2</sub><sup>2+</sup> inside the
binding pocket of AC1106 could be found. Rather, the addition of
methyl viologen
was found to result in the displacement of AC1106-bound
Ru(dmbpy)(bpy)<sub>2</sub><sup>2+</sup> from the antibody
binding site.