10.1021/acs.jmedchem.6b00869.s002 Alan G. Hendrick Alan G. Hendrick Ilka Müller Ilka Müller Henriëtte Willems Henriëtte Willems Philip M. Leonard Philip M. Leonard Steve Irving Steve Irving Richard Davenport Richard Davenport Takashi Ito Takashi Ito Jenny Reeves Jenny Reeves Susanne Wright Susanne Wright Vivienne Allen Vivienne Allen Stephen Wilkinson Stephen Wilkinson Helen Heffron Helen Heffron Richard Bazin Richard Bazin Jennifer Turney Jennifer Turney Philip J. Mitchell Philip J. Mitchell Identification and Investigation of Novel Binding Fragments in the Fatty Acid Binding Protein 6 (FABP6) American Chemical Society 2016 surface plasmon resonance crystal structure Fatty SPR program SAR Binding Protein 6 fragment hits Novel Binding Fragments ligand taurocholate fragment-based drug discovery FABP 6 drug discovery target Several hits 2016-08-08 00:00:00 Dataset https://acs.figshare.com/articles/dataset/Identification_and_Investigation_of_Novel_Binding_Fragments_in_the_Fatty_Acid_Binding_Protein_6_FABP6_/3644595 Fatty acid binding protein 6 (FABP6) is a potential drug discovery target, which, if inhibited, may have a therapeutic benefit for the treatment of diabetes. Currently, there are no published inhibitors of FABP6, and with the target believed to be amenable to fragment-based drug discovery, a structurally enabled program was initiated. This program successfully identified fragment hits using the surface plasmon resonance (SPR) platform. Several hits were validated with SAR and were found to be displaced by the natural ligand taurocholate. We report the first crystal structure of human FABP6 in the unbound form, in complex with cholate, and with one of the key fragments.