10.1021/bi982976i.s001
Andreas Janshoff
Andreas
Janshoff
Dennis T. Bong
Dennis T.
Bong
Claudia Steinem
Claudia
Steinem
John E. Johnson
John E.
Johnson
M. Reza Ghadiri
M. Reza
Ghadiri
An Animal Virus-Derived Peptide Switches Membrane Morphology: Possible
Relevance to Nodaviral Transfection Processes<sup>†</sup>
American Chemical Society
1999
peptide
interdigitated gel phase
membrane
capsid protein cleavage product
formation
bilayer
RNA
gel phase lipids
fluorescence spectroscopy
acyl chain interdigitated lipid domains
γ 1
FHV
DPPC
flock house virus
scanning force microscopy
1999-04-02 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/An_Animal_Virus-Derived_Peptide_Switches_Membrane_Morphology_Possible_Relevance_to_Nodaviral_Transfection_Processes_sup_sup_/3588807
The N-terminal domain of the capsid protein cleavage product of the flock house virus (FHV)
consists of 21 residues and forms an amphipathic α-helix, which is thought to play a crucial role in
permeabilizing biological membranes for RNA translocation in the host cell. We have found that the Met
→ Nle variant of this domain (denoted here as γ<sub>1</sub>) efficiently induces the formation of the interdigitated
gel phase (L<sub>β</sub>I) of 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phosphatidylcholine (DPPC) bilayers. In situ scanning
force microscopy of solid supported bilayers and fluorescence spectroscopy of peptide-treated DPPC vesicles
provide evidence for the formation of acyl chain interdigitated lipid domains. It could be shown by
fluorescence spectroscopy that the peptide inserts in the DPPC matrix above the main transition temperature
of the lipid, while the formation of domains with decreased thickness occurs after the sample is cooled
to 25 °C. The orientation and secondary structure of the peptide in lipid bilayers were investigated using
attenuated total reflectance infrared (ATR-IR) and circular dichroism (CD) spectroscopy. These results
enabled us to formulate a mechanistic model for the peptide-mediated induction of interdigitation in DPPC
bilayers. Moreover, the membrane activity of γ<sub>1</sub> with gel phase lipids established in this study may have
further implications for the infection strategy adopted by simple RNA viruses.