High-Density Fiber-Optic Genosensor Microsphere Array Capable of Zeptomole Detection Limits Jason R. Epstein Myoyong Lee David R. Walt 10.1021/ac0156619.s001 https://acs.figshare.com/articles/journal_contribution/High-Density_Fiber-Optic_Genosensor_Microsphere_Array_Capable_of_Zeptomole_Detection_Limits/3582816 The detection limit of a fiber-optic microsensor array was investigated for simultaneous detection of multiple DNA sequences. A random array composed of oligonucleotide-functionalized 3.1-μm-diameter microspheres on the distal face of a 500-μm etched imaging fiber was monitored for binding to fluorescently labeled complementary DNA sequences. Inherent sensor redundancy in the microarray allows the use of multiple microspheres to increase the signal-to-noise ratio, further enhancing the detection capabilities. Specific hybridization was observed for each of three sequences in an array yielding a detection limit of 10<sup>-21</sup> mol (∼600 DNA molecules). 2002-03-08 00:00:00 array microsphere DNA sequences detection limit Zeptomole Detection Limits Inherent sensor redundancy