High-Density Fiber-Optic Genosensor Microsphere
Array Capable of Zeptomole Detection Limits
Jason R. Epstein
Myoyong Lee
David R. Walt
10.1021/ac0156619.s001
https://acs.figshare.com/articles/journal_contribution/High-Density_Fiber-Optic_Genosensor_Microsphere_Array_Capable_of_Zeptomole_Detection_Limits/3582816
The detection limit of a fiber-optic microsensor array was
investigated for simultaneous detection of multiple DNA
sequences. A random array composed of oligonucleotide-functionalized 3.1-μm-diameter microspheres on the distal face of a 500-μm etched imaging fiber was monitored
for binding to fluorescently labeled complementary DNA
sequences. Inherent sensor redundancy in the microarray
allows the use of multiple microspheres to increase the
signal-to-noise ratio, further enhancing the detection
capabilities. Specific hybridization was observed for each
of three sequences in an array yielding a detection limit
of 10<sup>-21</sup> mol (∼600 DNA molecules).
2002-03-08 00:00:00
array
microsphere
DNA sequences
detection limit
Zeptomole Detection Limits
Inherent sensor redundancy