Deep-Red and Near-Infrared Xanthene Dyes for Rapid Live Cell Imaging Guangle Niu Weimin Liu Bingjiang Zhou Hongyan Xiao Hongyan Zhang Jiasheng Wu Jiechao Ge Pengfei Wang 10.1021/acs.joc.6b00981.s002 https://acs.figshare.com/articles/dataset/Deep-Red_and_Near-Infrared_Xanthene_Dyes_for_Rapid_Live_Cell_Imaging/3571239 In this work, two xanthene dyes (<b>H-hNR</b> and <b>TF-hNR</b>) have been synthesized by a convenient and efficient method. These two dyes exhibited deep-red and near-infrared emissions, high fluorescence quantum yields, and good photostability. Their structure–optical properties were investigated by X-ray crystal structure analysis and density functional theory calculations. Live cell imaging data revealed that <b>H-hNR</b> and <b>TF-hNR</b> could rapidly stain both A549 and HeLa cells with low concentrations. The excellent photophysical and imaging properties render them as promising candidates for use in live cell imaging. 2016-08-03 00:00:00 cell imaging Live cell imaging data xanthene dyes imaging properties HeLa cells theory calculations Rapid Live Cell Imaging fluorescence quantum yields