10.1021/acscombsci.6b00039.s001
Kevin
J. Fisher
Kevin
J.
Fisher
Jeremy A. Turkett
Jeremy A.
Turkett
Ashley E. Corson
Ashley E.
Corson
Kevin L. Bicker
Kevin L.
Bicker
Peptoid Library Agar Diffusion (PLAD) Assay for the
High-Throughput Identification of Antimicrobial Peptoids
American Chemical Society
2016
Peptoid Library Agar Diffusion
peptoid library agar diffusion
method
ESKAPE
Antimicrobial Peptoids Rapid emergence
strand
PLAD
antimicrobial peptoids
2016-05-17 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Peptoid_Library_Agar_Diffusion_PLAD_Assay_for_the_High-Throughput_Identification_of_Antimicrobial_Peptoids/3405208
Rapid emergence of antimicrobial
resistant organisms necessitates
equally rapid methods for the development of new antimicrobial compounds.
Of recent interest have been mimics of antimicrobial peptides known
as antimicrobial peptoids, which exhibit similar potency to the former
but with improved proteolytic stability. Presented herein is a high-throughput
method to screen libraries of antimicrobial peptoids immobilized on
beads embedded into solid media. Termed the peptoid library agar diffusion
(PLAD) assay, this assay allows for individual chemical manipulation
of two identical peptoid strands. One strand can be released to diffuse
out from a solid support bead and interact with the microorganism
during screening. The other strand can be cleaved after screening
from beads showing strong antimicrobial activity and analyzed by mass
spectrometry to deconvolute the structure of the peptoid. This method
was applied to a small library of peptoids to identify an antimicrobial
peptoid with modest efficacy against the ESKAPE pathogens.