Electrokinetic Trapping and Concentration Enrichment of DNA in a
Microfluidic Channel
Jinhua Dai
Takashi Ito
Li Sun
Richard M. Crooks
10.1021/ja0374776.s001
https://acs.figshare.com/articles/media/Electrokinetic_Trapping_and_Concentration_Enrichment_of_DNA_in_a_Microfluidic_Channel/3361015
We report a simple and efficient method for enriching the concentration of charged analytes within microfluidic channels. The method relies on exerting spatial control over the electrokinetic velocity of an analyte. Specifically, the electroosmotic (eo) velocity of the buffer solution in one region of the microfluidic system opposes the electrophoretic (ep) velocity of the analyte in the other region. This results in ep transport of DNA to the location where the ep and eo velocities are equal and opposite. Accumulation of the analyte occurs at this location. This enrichment method is conceptually distinct from field-amplification stacking, isotachophoresis, micelle sweeping, size exclusion, and other methods that have been previously reported. The method requires no complex microfabricated structures, no special manipulation of the solvent, and the enriched analyte remains in solution rather than being captured on a solid support. A concentration enrichment factor of 800 can be achieved for 20mer DNA in a fluidic channel having dimensions of 100 μm × 25 μm × 5 mm. The time required to achieve this level of enrichment is 300 s, and the enriched zone has a minimum width of 100 μm.
2003-10-29 00:00:00
Microfluidic Channel
fluidic channel
eo velocities
concentration enrichment factor
analyte
enrichment method
size exclusion
location
Electrokinetic Trapping
100 μ m
microfluidic system
electrokinetic velocity
microfluidic channels
20 mer DNA
microfabricated structures
buffer solution
Concentration Enrichment
ep transport
region