Oxenoid, Kirill Kim, Hak Jun Jacob, Jaison Sönnichsen, Frank D. Sanders, Charles R. NMR Assignments for a Helical 40 kDa Membrane Protein Backbone nuclear magnetic resonance (NMR) assignments were achieved for diacylglycerol kinase (DAGK) in detergent micelles. DAGK is a homotrimeric integral membrane protein comprised of 121 residue subunits, each having three transmembrane segments. Assignments were made using TROSY-based pulse sequences. DAGK was found to be an almost exclusively helical protein. This work points to the feasibility of both solving the structure of DAGK using solution NMR methods and using NMR as a primary tool in structural studies of other helical integral membrane proteins of similar size and complexity. homotrimeric integral membrane protein;solution NMR methods;helical protein;DAGK;helical integral membrane proteins;work points;Helical 40 kDa Membrane Protein Backbone;121 residue subunits;NMR Assignments;diacylglycerol kinase;detergent micelles;transmembrane segments 2004-04-28
    https://acs.figshare.com/articles/journal_contribution/NMR_Assignments_for_a_Helical_40_kDa_Membrane_Protein/3341650
10.1021/ja049916m.s001